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LAG-3 (human) monoclonal antibody (11E3)

 
ALX-804-805-C100 100 µg 486.00 USD
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Product Specification

Alternative Name:Lymphocyte activation gene-3, FDC protein, CD223
 
Clone:11E3
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant human LAG-3 (lymphocyte activation gene-3).
 
UniProt ID:P18627
 
Source:Purified from the hybridoma culture supernatant by Protein G Fast Flow Column.
 
Species reactivity:Human
Monkey
 
Specificity:Recognizes the first N-terminal D1 domain of LAG-3.
 
Applications:ELISA, ICC, IHC (FS), IP, WB, FUNC
 
Recommended Dilutions/Conditions:ELISA (capture)
Immunoprecipitation (10µg/ml)
Western Blot (5µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Formulation:Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:The lymphocyte activation gene-3 (LAG-3, CD223), a member of the immunoglobulin superfamily (IgSF) related to CD4, binds to the major histocompatibility complex (MHC) class II molecules but with higher affinity than CD4. Several alternative mRNA splice-variants of human LAG-3 have been described, two of them encoding potential secreted forms: LAG-3V1 (i.e. the D1-D2 domains of the protein, 36 kDa) and LAG-3V3 (D1-D3, 52 kDa). The longer form was detected by ELISA in the serum of healthy individuals as well as of tuberculosis patients with a favorable outcome. LAG-3 expression by T cell clones correlated with IFN-γ production, and hence soluble LAG-3 has been suggested as a serological marker of Th1 responses.
 

Product Literature References

Elevated expression of LAG-3, but not PD-1, is associated with impaired iNKT cytokine production during chronic HIV-1 infection and treatment : J.A. Juno, et al.; Retrovirology 12, 17 (2015), Application(s): ELISA, Abstract; Full Text
A soluble lymphocyte activation gene-3 (sLAG-3) protein as a prognostic factor in human breast cancer expressing estrogen or progesterone receptors: F. Triebel, et al.; Cancer Lett. 235, 147 (2006), Abstract;
Characterization of the major histocompatibility complex class II binding site on LAG-3 protein: B. Huard, et al.; PNAS 94, 5744 (1997), Abstract; Full Text
Cellular expression and tissue distribution of the human LAG-3-encoded protein, an MHC class II ligand: B. Huard, et al.; Immunogenetics 39, 213 (1994), Abstract;

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