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NLRP1/NALP1 (human) monoclonal antibody (Nalpy1-4)

 
ALX-804-803-C100 100 µg 323.00 USD
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Product Specification

Alternative Name:NACHT-, LRR- and PYD-containing protein 1, DEFCAP, NAC, CARD7, Caspase recruitment domain-containing protein 7
 
Clone:Nalpy1-4
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant human NLRP1/NALP1 (NACHT-, LRR- and PYD-containing protein 1) (pyrin domain).
 
UniProt ID:Q9C000
 
Source:Purified from concentrated hybridoma tissue culture supernatant.
 
Species reactivity:Human
 
Specificity:Recognizes the pyrin domain (PYD) of NLRP1/NALP1.
 
Crossreactivity:Does not cross-react with mouse NLRP1/NALP1.
 
Applications:ICC, IHC (FS), IHC (PS), IP, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (1:1,000)
Immunohistochemistry (paraffin sections, microwave treatment)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Positive Control:For Positive Control (Western blot) see Prod. No. ALX-840-603.
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
804-803
Figure: Western blot analysis of the time course of assembly of the inflammasome using MAb to NLRP1/NALP1 (Nalpy1-4) in THP-1 macrophages.Method: Assembly of the inflammasome was induced by shifting the temperature to 30°C after hypotonic lysis. THP-1 cell extracts were immunoprecipitated using PAb to Asc (AL177) (Prod. No. ALX-210-905) and run on SDS-PAGE. NLRP1/NALP1 was detected by MAb to NALP1 (Nalpy1-4) (Prod. No. ALX-804-803) at 1:1’000 dilution. Anti-mouse IgG coupled horse radish peroxidase was used at 1:5’000 dilution for ECL detection. For more information about the "time course assembly of inflammasome" method see F. Martinon; Mol. Cell. 10, 417 (2002).
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Figure: Detection of NLRP1/NALP1 in 293T cells transfected with a human NLRP1/NALP1 expression plasmid. Left: Phase contrast. Right: Staining with MAb to NLRP1/NALP1 (Nalpy1-4).Method: 293T cells transfected with full length human NLRP1/NALP1 were fixed with 4% paraformaldehyde and stained with 1µg/ml MAb to NLRP1/NALP1 (Nalpy1-4) (Prod. No. ALX-804-803) and then Alexa 488 (1:300 dilution).
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Product Literature References

Inflammasome components NALP 1 and 3 show distinct but separate expression profiles in human tissues suggesting a site-specific role in the inflammatory response: J.A. Kummer, et al.; J. Histochem. Cytochem. 55, 443 (2007), Abstract;

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