NLRP1/NALP1 (human), mAb (Nalpy1-4)



ALX-804-803-C100	100 µg	315.00 USD

Product Specification

Alternative Name:	NACHT-, LRR- and PYD-containing protein 1, DEFCAP, NAC, CARD7, Caspase recruitment domain-containing protein 7

Concentration:	1mg/ml

Purity:	≥95% (SDS-PAGE)

Formulation:	Liquid. In PBS containing 0.02% sodium azide.

Clone:	Nalpy1-4

Isotype:	Mouse IgG1

Immunogen:	Recombinant human NLRP1/NALP1 (NACHT-, LRR- and PYD-containing protein 1) (pyrin domain).

Source/Host:	Purified from concentrated hybridoma tissue culture supernatant.

Specificity:	Recognizes the pyrin domain (PYD) of human NLRP1/NALP1. Does not cross-react with mouse NLRP1/NALP1.

Application:	Immunocytochemistry (1:1'000) Immunohistochemistry (frozen sections, paraffin sections (microwave treatment)) Immunoprecipitation Western Blot (1:1'000)

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 1 year after receipt when stored at -20°C.

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Positive Control:	For Positive Control (Western blot) see Prod. No. ALX-840-603.

Figure: Western blot analysis of the time course of assembly of the inflammasome using MAb to NLRP1/NALP1 (Nalpy1-4) in THP-1 macrophages.Method: Assembly of the inflammasome was induced by shifting the temperature to 30°C after hypotonic lysis. THP-1 cell extracts were immunoprecipitated using PAb to Asc (AL177) (Prod. No. ALX-210-905) and run on SDS-PAGE. NLRP1/NALP1 was detected by MAb to NALP1 (Nalpy1-4) (Prod. No. ALX-804-803) at 1:1’000 dilution. Anti-mouse IgG coupled horse radish peroxidase was used at 1:5’000 dilution for ECL detection. For more information about the "time course assembly of inflammasome" method see F. Martinon; Mol. Cell. 10, 417 (2002).

Figure: Detection of NLRP1/NALP1 in 293T cells transfected with a human NLRP1/NALP1 expression plasmid. Left: Phase contrast. Right: Staining with MAb to NLRP1/NALP1 (Nalpy1-4).Method: 293T cells transfected with full length human NLRP1/NALP1 were fixed with 4% paraformaldehyde and stained with 1µg/ml MAb to NLRP1/NALP1 (Nalpy1-4) (Prod. No. ALX-804-803) and then Alexa 488 (1:300 dilution).

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Product Literature References

Inflammasome components NALP 1 and 3 show distinct but separate expression profiles in human tissues suggesting a site-specific role in the inflammatory response: J.A. Kummer, et al.; J. Histochem. Cytochem. 55, 443 (2007), Abstract;