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TACI (mouse) monoclonal antibody (8F10)

 
ALX-804-800-C100 100 µg 386.00 USD
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Product Specification

Alternative Name:Transmembrane activator and CAML interactor, TNFRSF 13B, CD267
 
Clone:8F10
 
Host:Rat
 
Isotype:IgG2a
 
Immunogen:Rat RBL cells expressing mouse TACI.
 
UniProt ID:Q9ET35
 
Source:Purified from concentrated hybridoma tissue culture supernatant.
 
Species reactivity:Mouse
 
Applications:Flow Cytometry, IHC (FS), FUNC
 
Application Notes:Functional Application: inhibition.
 
Formulation:Liquid. In PBS.
 
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
804-800
Method: HEK 293T  cells were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the extracellular domain of mouse TACI (thick line). Cells (5 x 105) were incubated on ice for 30 min in 50 µl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1µg/ml of rat anti-mTACI 8F10 MAb. After washing in FACS buffer, R-PE-conjugated antibody to rat IgG (Prod. No. ALX-211-053) was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.
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Product Literature References

CD45 links the B cell receptor with cell survival and is required for the persistence of germinal centers: N.D. Huntington, et al.; Nat. Immunol. 7, 190 (2006), Abstract;
TACI-BLyS signaling via B-cell-dendritic cell cooperation is required for naive CD8+ T-cell priming in vivo: Y. Diaz-de-Durana, et al.; Blood 107, 594 (2006), Abstract;
B Cell-Activating Factor Belonging to the TNF Family (BAFF)-R Is the Principal BAFF Receptor Facilitating BAFF Costimulation of Circulating T and B Cells: L.G. Ng, et al.; J. Immunol. 173, 807 (2004), Abstract;
TNF deficiency fails to protect BAFF transgenic mice against autoimmunity and reveals a predisposition to B cell lymphoma: M. Batten, et al.; J. Immunol. 172, 812 (2004), Abstract;

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