DLK1 (human), mAb (PF299-1)



ALX-804-755-C050	50 µg	226.00 USD

ALX-804-755-C100	100 µg	278.00 USD

Product Specification

Alternative Name:	Protein delta homolog 1, pG2, Fetal antigen 1, FA1

Concentration:	1mg/ml

Formulation:	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.

Clone:	PF299-1

Isotype:	Mouse IgG1

Immunogen:	Recombinant human DLK1 (extracellular domain).

Specificity:	Recognizes human DLK1.

Application:	ELISA (direct and indirect: 1:2’000-1:10’000) Flow Cytometry (1:100-1:500) Immunohistochemistry (paraffin sections, 2.5µg/ml) Western Blot (1:2’000-1:10’000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Use/Stability:	Stable for up to 6 months when stored at -20°C.

Figure 1: Western blot analysis of human Dlk1 using MAb to Dlk1 (human) (PF299-1) (Prod. No. ALX-804-755) at dilution 1:2’000.A.1. Dlk1 (human):Fc (human) (rec.) (Prod. No. ALX-201-427)2. Dlk1 (human) (full length) transfected cell lysate (HEK 293)3. Mock transfected cell lysate (HEK 293)B.1. Dlk1 (human):Fc (human) (rec.) (Prod. No. ALX-201-427)2. Dlk1 (mouse):Fc (human) (rec.) (Prod. No. ALX-201-416)3. DNER (Extracellular Domain) (human):Fc (human) (rec.) (Prod. No. ALX-201-428)4. DLL1 (human):Fc (human) (rec.) (Prod. No. ALX-201-425)5. DLL3 (human):Fc (human) (rec.) (Prod. No. ALX-201-422)6. DLL4 (human):Fc (human) (rec.) (Prod. No. ALX-201-386)7. Jagged-1 (human):Fc (human) (rec.) (Prod. No. ALX-201-390)8. FGF-23 (human):Fc (human) (rec.) (Prod. No. ALX-201-418)

Figure 2: Immunohistochemical staining of DLK1 with DLK1 (human), mAb (PF299-1) (Prod. No. ALX-804-755) in Placenta, Villi (1:500 dilution).
Methods: This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The antibody was serially diluted and tested at a range of concentrations on approximately 22 different human formalin-fixed, paraffin archival tissues, and positive and negative tissues were scored and compared to the published literature on the expression and function of the gene. A representative image from a positively stained tissue is included in this report accompanied by the pathologist's report of the IHC localization pattern for that tissue. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

Figure 3: Flow Cytometry. DLK1(full length)-transfected HEK 293 cells were stained significantly by MAb to Dlk1 (human) (PF299-1) (Prod. No. ALX-804-755).

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