DLK1 (human), mAb (PF13-3)



ALX-804-754-C050	50 µg	226.00 USD

ALX-804-754-C100	100 µg	278.00 USD

Product Specification

Alternative Name:	Protein delta homolog 1, pG2, Fetal antigen 1, FA1

Concentration:	~1mg/ml

Formulation:	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.

Clone:	PF13-3

Isotype:	Mouse IgG1

Immunogen:	Recombinant human DLK1 (extracellular domain).

Specificity:	Recognizes human DLK1. Does not cross-react with mouse DLK1.

Application:	ELISA (direct and indirect: 1:2’000-1:10’000) Flow Cytometry (1:100-1:500) Immunohistochemistry (1:200-1:1’000) Western Blot (1:2’000-1:10’000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Use/Stability:	Stable for up to 6 months when stored at -20°C.

Figure 1: Western blot analysis of human Dlk1 using MAb to Dlk1 (human) (PF13-3) (Prod. No. ALX-804-754) at dilution 1:2’000.A.1. Dlk1 (human):Fc (human) (rec.) (Prod. No. ALX-201-427)2. Dlk1 (human) (full length) transfected cell lysate (HEK 293)3. Mock transfected cell lysate (HEK 293)B.1. Dlk1 (human):Fc (human) (rec.) (Prod. No. ALX-201-427)2. Dlk1 (mouse):Fc (human) (rec.) (Prod. No. ALX-201-416)3. DNER (Extracellular Domain) (human):Fc (human) (rec.) (Prod. No. ALX-201-428)4. DLL1 (human):Fc (human) (rec.) (Prod. No. ALX-201-425)5. DLL3 (human):Fc (human) (rec.) (Prod. No. ALX-201-422)6. DLL4 (human):Fc (human) (rec.) (Prod. No. ALX-201-386)7. Jagged-1 (human):Fc (human) (rec.) (Prod. No. ALX-201-390)8. FGF-23 (human):Fc (human) (rec.) (Prod. No. ALX-201-418)

Figure 2: Immunohistochemical staining of Dlk1 in human tissue using MAb to Dlk1 (human) (PF13-3) (Prod. No. ALX-804-754) at dilution 1:500. Inset: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human umbilical cord showing cytoplasmic staining (200x, brown color).

Figure 3: Flow Cytometry. DLK1(full length)-transfected HEK 293 cells were stained significantly by MAb to Dlk1 (human) (PF13-3) (Prod. No. ALX-804-754).

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