DLK1 (mouse), mAb (PF105B)



ALX-804-742-C050	50 µg	226.00 USD

ALX-804-742-C100	100 µg	278.00 USD

Product Specification

Alternative Name:	Protein delta homolog 1 , Pref-1, Preadipocyte factor 1, Fetal antigen 1, FA1

Concentration:	~1mg/ml

Formulation:	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.

Clone:	PF105B

Isotype:	Rat IgG2a

Immunogen:	Recombinant mouse Dlk1 (extracellular domain).

Specificity:	Recognizes mouse Dlk1. Does not cross-react with human DLK1.

Application:	ELISA (direct and indirect: 1:2’000-1:10’000) Western Blot (1:2’000-1:10’000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Use/Stability:	Stable for up to 6 months when stored at -20°C.

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Figure: Western blot analysis of mouse Dlk1.
Method: Detection with DLK1 (mouse), mAb (PF105B) (Prod. No. ALX-804-742) using 1: 2,000 dilution.
A) 1. DLK1 (mouse):Fc (human) (rec.) (Prod. No. ALX-201-416)
2. DLK1 (human):Fc (human), (rec.) (Prod. No. ALX-201-427)
3. DLL1 (mouse):Fc (human) (rec.) (Prod. No. ALX-201-455)
4. DLL4 (mouse):Fc (human), (rec.) (Prod. No. ALX-201-453)
5. Jagged-1 (mouse):Fc (human) (rec.) (Prod. No. ALX-201-463)
6. An unrelated protein (Fc fusion)
B) 1. DLK1 (mouse):Fc (human) (rec.) (Prod. No. ALX-201-416)
2. DLK1 (human) (ECD) (FLAG)
3. DLL1 (mouse):Fc (human) (rec.) (Prod. No. ALX-201-455)
4. 3T3L1 cell lysate

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