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IL-33 monoclonal antibody (IL33068A)

 
ALX-804-727-C050 50 µg 240.00 USD
 
ALX-804-727-C100 100 µg 290.00 USD
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Product Specification

Alternative Name:Interleukin-33, IL-1F11, NF-HEV
 
Clone:IL33068A
 
Host:Mouse
 
Isotype:IgG2b
 
Immunogen:Recombinant human IL-33 (interleukin-33).
 
UniProt ID:O95760
 
Gene/Protein Identifier:90865 (Entrez GeneID), 608678 (OMIM)
 
Species reactivity:Human
 
Crossreactivity:Weakly cross-reacts with mouse IL-33.
 
Applications:ELISA, WB
 
Recommended Dilutions/Conditions:ELISA (direct and indirect, 1:2,000-1:5,000)
Western Blot (1:2,000-1:5,000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Formulation:Liquid. In PBS, pH 7.4. Contains no preservatives.
 
Use/Stability:Stable for 1-2 weeks when stored at +4°C.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
804-727
Figure: Western blot analysis of human IL-33 using MAb to IL-33 (IL33068A) (Prod. No. ALX-804-727) at 1:2000 dilution. 1. Recombinant His-tagged human IL-33 (Prod. No. ALX-201-350).2. Recombinant FLAG®-tagged human IL-33 (Prod. No. ALX-201-363).3. Recombinant FLAG®-tagged mouse IL-33. 4. Other His-tagged protein (negative control).
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804-727

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