ANGPTL3 (human), mAb (Kairos-37)



ALX-804-724-C050	50 µg	226.00 USD

ALX-804-724-C100	100 µg	278.00 USD

Product Specification

Alternative Name:	Angiopoietin-like protein 3, Angiopoietin-5, ANG-5

Concentration:	1mg/ml

Formulation:	Liquid. In PBS, pH 7.4. Contains no preservatives.

Clone:	Kairos-37

Isotype:	Mouse IgG1

Immunogen:	Recombinant human ANGPTL3 (angiopoietin-like protein 3; ANG-5).

Specificity:	Recognizes human ANGPTL3 and the fibrinogen-like domain (FLD) of human ANGPTL3. Detects a band of ~68kDa and a cleaved band of ~35kDa by Western blot. Does not cross-react with other ANGPTL family proteins.

Application:	ELISA (direct and indirect: 1:1’000-1:5’000) Western Blot (1:2’000-1:5’000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 6 months after receipt when stored at -20°C.

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Figure A: Western blot analysis using MAb to ANGPTL3 (human) (Kairos-37) (Prod. No. ALX-804-724) at 1:2’000 dilution. 1. FLAG^®-tagged ANGPTL1 (human) (rec.). 2. FLAG^®-tagged ANGPTL2 (human) (rec.). 3. FLAG^®-tagged ANGPTL3 (human) (rec.) (Prod. No. ALX-201-359).4. FLAG^®-tagged ANGPTL4 (Fibrinogen-like Domain) (human) (rec.)(Prod. No. ALX-201-379). 5. FLAG^®-tagged ANGPTL4 (Coiled-coil Domain) (human) (rec.) (Prod. No. ALX-201-373).6. FLAG^®-tagged ANGPTL6 (human) (rec.) (Prod. No. ALX-201-337).7. FLAG^®-tagged ANGPTL7 (human) (rec.) (Prod. No. ALX-201-368).8. FLAG^®-tagged RBP4 (mouse) (rec.) (negative control) (Prod. No. ALX-201-353).

Figure B: Western blot analysis using MAb to ANGPTL3 (human) (Kairos-37) (Prod. No. ALX-804-724) at 1:2’000 dilution. 1. FLAG^®-tagged ANGPTL3 (human) (rec.) (Prod. No. ALX-201-359). 2. FLAG^®-tagged ANGPTL3 (Coiled-coil Domain) (human) (rec.) (Prod. No. ALX-201-378). 3. FLAG^®-tagged ANGPTL3 (Fibrinogen-like Domain) (human) (rec.) (Prod. No. ALX-201-380).

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