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Leptin (human) monoclonal antibody (HLEP 155)

 
ALX-804-702-C100 100 µg 280.00 USD
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Product Specification

Alternative Name:OB gene product
 
Clone:HLEP 155
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant human leptin.
 
UniProt ID:P41159
 
Species reactivity:Human
 
Specificity:Recognizes both natural and recombinant leptin.
 
Crossreactivity:Does not cross-react with mouse or rat leptin.
 
Applications:ELISA, WB
 
Recommended Dilutions/Conditions:ELISA (direct and indirect, 1:5,000-1:10,000)
Western Blot (1:2,000-1:10,000 using ECL; suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~16kDa by Western blot.
 
Formulation:Liquid. In PBS, pH 7.4. Contains no preservatives.
 
Handling:After receipt, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
804-702
Figure: Western blot analysis of recombinant Leptin with HLEP155 (Prod. No. ALX-804-702) at 1:5,000 dilution. Lane 1) Recombinant human Leptin protein. Lane 2) Human Leptin-Fc fusion protein. 
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804-702

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Leptin Monoclonal antibody
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