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Leptin (rat) monoclonal antibody (RLEP 227)

 
ALX-804-701-C050 50 µg 230.00 USD
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Product Specification

Alternative Name:OB gene product
 
Clone:RLEP 227
 
Host:Mouse
 
Isotype:IgG2a
 
Immunogen:Recombinant rat leptin.
 
UniProt ID:P50596
 
Species reactivity:Rat
 
Specificity:Recognizes both natural and recombinant leptin.
 
Crossreactivity:Does not cross-react with human or mouse leptin.
 
Applications:ELISA, WB
 
Recommended Dilutions/Conditions:ELISA (direct and indirect, 1:5,000-1:10,000)
Western Blot (1:5,000-1:10,000 using ECL; suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~16kDa by Western blot.
 
Formulation:Liquid. In PBS, pH 7.4. Contains no preservatives.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
804-701
Figure: Western blot analysis of recombinant rat leptin with MAb to Leptin (rat) (RLEP 227) (Prod. No. ALX-804-701) at 1:5’000 dilution.1. Recombinant rat leptin.2. Recombinant mouse leptin.3. Recombinant human leptin.
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804-701

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