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[K20-trimethyl]Histone H4 monoclonal antibody (6F8-D9)

 
ALX-804-676-L001 1 ml 350.00 USD
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Product Specification

Alternative Name:Trimethyl-histone H4 (Lys20), H4K20me3
 
Clone:6F8-D9
 
Host:Mouse
 
Isotype:IgG1 κ
 
Immunogen:Branched peptide of the sequence (AKRHRK(Me)3VLRDN)2-KC, corresponding to aa 15-25 of human histone H4.
 
UniProt ID:P62805
 
Species reactivity:Human
 
Specificity:Recognizes human K20 trimethylated histone H4.
 
Crossreactivity:Weakly cross-reacts with human K20 dimethylated histone H4. Predicted to cross-react with K20 di-/trimethylated histone H4 of all mammals, Xenopus, Drosophila and Arabidopsis. Does not cross-react with methylated histone H3.
 
Applications:ELISA, WB
ChIP, Dot Blot
 
Application Notes:Western Blot (with endogenous protein)
 
Formulation:Liquid. Hybridoma supernatant containing 0.02% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
804-676 1
Figure 1: Peptide dot blot: 50pmol or 10pmol of the indicated peptides corresponding to aa 12-31 of histone H4 and containing either unmethylated, or mono-, di-, or trimethylated lysine 20 were spotted onto a PVDF membrane. After blocking for 60 min. in 3% NFDM in PBS-T, the membrane was incubated for 60 min. at RT with anti-H4K20me3 MAb (6F8-D9) (Prod. No. ALX-804-676),  dilution 1:20, and processed for Western blotting.
804-676 2
Figure 2: Western Blot against HeLa histones (approx. 2μg loaded per lane) with anti-H4K20me3 MAb (6F8-D9) (Prod. No. ALX-804-676) at the indicated dilutions; 30 sec exposure.
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