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[K20-monomethyl]Histone H4 monoclonal antibody (5E10-D8)

ALX-804-674-L001 1 ml 360.00 USD
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Product Specification

Alternative Name:H4K20me1, Monomethyl-histone H4 (Lys20)
Isotype:IgG1 κ
Immunogen:Branched peptide of the sequence (AKRHRK(Me)VLRDN)2-KC, corresponding to aa 15-25 of human histone H4.
UniProt ID:P62805
Species reactivity:Human
Specificity:Recognizes human K20 monomethylated histone H4.
Crossreactivity:Predicted to cross-react with K20 monomethylated histone H4 of all mammals, Xenopus, Drosophila and Arabidopsis. Does not cross-react with unmethylated or di-/trimethylated histone H4 or with methylated histone H3.
Applications:ELISA, WB
ChIP, Dot Blot
Application Notes:Western Blot (with endogenous protein)
Formulation:Liquid. Hybridoma supernatant containing 0.02% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
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Figure 1: Peptide dot blot: 50pmol or 10pmol of the indicated peptides corresponding to aa 12-31 of histone H4 and containing either unmethylated, or mono-, di-, or trimethylated lysine 20 were spotted onto a PVDF membrane. After blocking for 60 min. in 3% NFDM in PBS-T, the membrane was incubated for 60 min. at RT with anti-H4K20me1 MAb (5E10-D8) (Prod. No. ALX-804-674), dilution 1:5, and processed for Western blotting.
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Figure 2: Western Blot against HeLa histones (approx. 2μg loaded per lane) with anti-H4K20me1 MAb (5E10-D8) (Prod. No. ALX-804-674) at the indicated dilutions, and, as control, a high concentration Fibercell supernatant of anti-H3K9me3 MAb (6F12-H4) (Prod. No. ALX-804-673) dilution 1:500, 90 sec exposure.
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General Literature References

Monomethylation of lysine 20 on histone H4 facilitates chromatin maturation: A.N. Scharf, et al.; Mol. Cell. Biol. 29, 57 (2009), Abstract;
Histone H4 lysine 20 monomethylation promotes transcriptional repression by L3MBTL1: N. Kalakonda, et al.; Oncogene 27, 4293 (2008), Abstract;
Aberrant monomethylation of histone H4 lysine 20 activates the DNA damage checkpoint in Drosophila melanogaster: A. Sakaguchi & R. Steward; J. Cell Biol. 176, 155 (2007), Abstract;
Histone H4-lysine 20 monomethylation is increased in promoter and coding regions of active genes and correlates with hyperacetylation: H. Talasz, et al.; J. Biol. Chem. 280, 38814 (2005), Abstract;
A switch in mitotic histone H4 lysine 20 methylation status is linked to M phase defects upon loss of HCF-1: E. Julien & W. Herr; Mol. Cell 14, 713 (2004), Abstract;
Methylation of histone H4 lysine 20 controls recruitment of Crb2 to sites of DNA damage: S.L. Sanders, et al.; Cell 119, 603 (2004), Abstract;

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