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Progerin (human) monoclonal antibody (13A4)

 
ALX-804-662-R200 200 µl 316.00 USD
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Product Specification

Alternative Name:Δ50 Lamin A
 
Clone:13A4
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Synthetic KLH-coupled peptide corresponding to aa 604-611 (G604AQSPQNC611) at the C-terminus of human progerin.
 
UniProt ID:Q6UYC3
 
Species reactivity:Human
 
Crossreactivity:Does not cross-react with endogenous prelamin A.
 
Applications:ICC, IP, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (1:10)
Immunoprecipitation (20µl/assay)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Formulation:Liquid. Neat cell culture supernatant containing 0.02% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
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Figure 1: Western blot analysis (10% PAGE) of whole cell lysate of HeLa cells ectopically expressing FLAG-tagged human progerin. The membrane was cut in strips and each strip was incubated separately with the following antibodies: Lane 1: anti-Lamin A/C antibody (N18) (1:2000), Lane 2: anti-FLAG-tag antibody (M2) (1:5000), Lane 3: MAb to Progerin (human) (13A4) (Prod. No. ALX-804-662) (1:5)
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Figure 2: Western blot analysis (7.5% PAGE) of whole cell lysate of T98G cells treated with FTI-277, a farnesyl transferase inhibitor, which causes accumulation of unprocessed lamin A (prelamin A). The membrane was cut in strips and each strip was incubated separately with the following antibodies: Lane 1: anti-lamin A/C antibody (N-18) (1:2000), Lane 2: anti-lamin A antibody (C-20) specific for prelamin A (1:500), Lane 3: MAb to Progerin (human) (13A4) (Prod. No. ALX-804-662) (1:5)
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Figure 3: Immunoprecipitation of progerin from lysates of Hutchinson-Gilford Progeria patient and control cells using 20μl of MAb to Progerin (human) (13A4) (Prod. No. ALX-804-662). The immunoprecipitates were separated by SDS-PAGE, blotted and incubated with MAb to Progerin (human) (13A4) (1:200). The asteriks labels the IgG band. Figure courtesy of Giovanna Lattanzi
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Figure 4: Immunocytochemistry of skin fibroblasts using MAb to Progerin (human) (13A4) (Prod. No. ALX-804-662) and DAPI. Only Hutchinson-Gilford Progeria Syndrome (HGPS) skin fibroblasts show staining.Figure courtesy of Giovanna Lattanzi
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Product Literature References

Low levels of the reverse transactivator fail to induce target transgene expression in vascular smooth muscle cells: N. Viceconte, et al.; PLoS One 9, e0104098 (2014), Application(s): Immunofluorescence on mouse aorta, Abstract; Full Text
Lamin A precursor induces barrier-to-autointegration factor nuclear localization: C. Capanni, et al.; Cell Cycle 9, 2600 (2010), Abstract;
Ageing-related chromatin defects through loss of the NURD complex: G. Pegoraro, et al.; Nat. Cell Biol. 11, 1261 (2009), Abstract;

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