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α-Synuclein (human) monoclonal antibody (5C2)

ALX-804-656-R100 100 µl 374.00 USD
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Product Specification

Immunogen:Recombinant human α-synuclein (aa 1-140).
UniProt ID:P37840
Species reactivity:Human
Specificity:Recognizes the NAC domain (aa 61-95) of human α-synuclein.
Applications:ELISA, WB
Recommended Dilutions/Conditions:Western Blot (1:500-1:2,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Protein G-affinity purified.
Formulation:Liquid. In PBS, pH 7.4, containing 0.02% thimerosal.
Use/Stability:Stable for one month when stored at 4°C.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Scientific Background:α-synuclein, an acidic neuronal protein of 140 aa, has been suggested to be implicated in the pathogenesis of Parkinson's disease and related neurodegenerative disorders, and to be an important regulatory component of vesicular transport in neuronal cells. The NAC (non-Aβ component of amyloid plaques) domain (aa 61-95) of α-synuclein is an highly amyloidogenic peptide which was first identified associated with senile plaques in the Alzheimer's disease brain.
α-Synuclein (human) monoclonal antibody (5C2) Western blot
Figure: Western blot analysis. The recombinant human synuclein family (α-, β- and γ-) and α-synuclein domains (1-60, 1-95, 61-140 and 96-140) proteins were resolved by SDS-PAGE, transferred to PVDF membrane and probed with MAb to α-Synuclein (human) (5C2) (Prod. No. ALX-804-656) (1:1’000). Proteins were visualized using a goat anti-mouse secondary antobody conjugated to HRP and an ECL detection system."
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α-Synuclein (human) monoclonal antibody (5C2) Western blot

General Literature References

Neurobiology of alpha-synuclein: K. Vekrellis, et al.; Mol. Neurobiol. 30, 1 (2004), (Review), Abstract;

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