Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

HERG1 (human) monoclonal antibody (A12)

 
ALX-804-652-R300 300 µl 453.00 USD
Do you need bulk/larger quantities?
 

Product Specification

Alternative Name:Erg1, Ether-a-go-go related protein 1, KCNH2, Voltage-gated potassium channel subfamily H member 2, Voltage-gated potassium channel Kv11.1
 
Clone:A12
 
Host:Mouse
 
Isotype:IgG2b
 
Immunogen:Synthetic peptide corresponding to aa 575-588 (E575QPHMDSRIGWLHN588) of the extracellular loop S5-P of HERG1 (human ether-a-go-go related protein 1).
 
UniProt ID:Q12809
 
Species reactivity:Human
 
Specificity:Recognizes the extracellular loop S5-P of HERG1.
 
Applications:Flow Cytometry, ICC, IHC (PS), IP
 
Recommended Dilutions/Conditions:Flow Cytometry (1:2-1:50)
Immunocytochemistry (1:200-1:1,000)
Immunohistochemistry (paraffin sections, 1:50-1:200)
Immunoprecipitation (3-5µl)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Formulation:Liquid. Sterile DMEM medium containing 10% bovine calf serum.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:The ether-a-go-go related gene family of K+ channels (HERG/Erg) comprises three members, HERG1/Erg1, HERG2/Erg2, HERG3/Erg3. HERG channel activity is relevant to tumor cell biology. It controls cell proliferation, especially in leukaemias. HERG channels are thus considered diagnostic and prognostic factors in human cancers, as well as targets for anti-neoplastic therapies.
 
804-652 1
Figure 1: Immunohistochemistry on colonic mucosa (left) and adenocarcinoma (right) using MAb to HERG1 (human) (A12) (Prod. No. ALX-804-652), performed without permeabilization.
804-652 2
Figure 2: FACS analysis of live intact HEK293-HERG1 transfected cells. Blue: MAb to HERG1 (human) (A12) (Prod. No. ALX-804-652) and goat anti-mouse (FITC)Green: Secondary antibody only.
804-652 3
Figure 3: Immunocytochemistry of HL-1 mouse atrial tumor cells using MAb to HERG1 (human) (A12) (Prod. No. ALX-804-652) (dilution: 1:25).Method: HL-1 cells were blocked with 1% BSA in PBS for 30 min on ice and then incubated with MAb to HERG1 (human) (A12) diluted 1:25 in 1% BSA in PBS for 2 hrs on ice. Cells were then fixed with 2% paraformaldehyde for 30 min at RT and blocked with 5% donkey serum in PBS-T (0.05% tween) for 30 min at RT and then with donkey anti-mouse alexa 488 secondary antibody for 1hr at RT.
Please mouse over
804-652 1 804-652 2 804-652 3

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis

RELATED PRODUCTS

By target:
HERG
Potassium channel
By biological activity:
HERG Monoclonal antibody
Potassium channel Monoclonal antibody
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Brochures
Essential Research Tools for Neurodegeneration & Neural Signaling
Essential Research Tools for Neurodegeneration & Neural Signaling
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,