ANGPTL3 (human), mAb (1D10)



ALX-804-635-R050	50 µl	131.00 USD

ALX-804-635-R100	100 µl	220.00 USD

Product Specification

Alternative Name:	Angiopoietin-like protein 3, Angiopoietin-5, ANG-5

Concentration:	1mg/ml

Purity Detail:	Protein G-affinity purified.

Purity:	≥95% (SDS-PAGE)

Formulation:	Liquid. In PBS, pH 7.4, containing 0.02% thimerosal.

Clone:	1D10

Isotype:	Mouse IgG1

Immunogen:	Recombinant human ANGPTL3 (angiopoietin-like protein 3) (aa 243-460).

Specificity:	Recognizes human ANGPTL3. Detects bands of ~64kDa (full-length) and ~36kDa (cleaved ANGPTL3) by Western blot.

Application:	ELISA Western Blot (1:500-1:2’000) Recommended starting dilution is 1:1’000. Optimal conditions must be determined individually for each application.

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Handling:	Avoid freeze/thaw cycles.

Figure: Western blot analysis of extracts of mouse liver tissue (80µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with MAb to ANGPTL3 (human) (1D10) (Prod. No. ALX-804-635) (1:1’000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Arrows indicate ANGPTL3 proteins. Upper is a full-length protein (~63kDa) and lower is a cleaved form of ANGPTL3 proteins (~35kDa) (see [2]).

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Product Literature References

Angiopoietin-like protein 3 mediates hypertriglyceridemia induced by the liver X receptor: T. Inaba, et al.; J. Biol. Chem. 278, 21344 (2003), Abstract; Full Text

Protein region important for regulation of lipid metabolism in angiopoietin-like 3 (ANGPTL3): ANGPTL3 is cleaved and activated in vivo: M. Ono, et al.; J. Biol. Chem. 278, 41804 (2003), Abstract; Full Text