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ERGIC-53 monoclonal antibody (G1/93)

ALX-804-602-C100 100 µg 370.00 USD
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Product Specification

Alternative Name:ER-Golgi intermediate compartment 53kDa protein, LMAN1
Immunogen:Human ERGIC-53 (ER-golgi intermediate compartment 53kDa protein) from Caco-2 cell golgi membrane fraction.
UniProt ID:P49257
Species reactivity:Human
Monkey, Porcine
Applications:ELISA, FC, ICC, IHC (FS), IP, WB
Recommended Dilutions/Conditions:Immunocytochemistry (1:1,000)
Immunoprecipitation (2µl)
Western Blot (1:250)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Affinity purified (HYDRA column) and gel filtrated (FPLC).
Reconstitution:Reconstitute with 100µl PBS containing 0.1% sodium azide.
Handling:Avoid freeze/thaw cycles.
Long Term Storage:-20°C
Scientific Background:The ER-Golgi intermediate compartment (ERGIC) marker ERGIC-53 is a mannose-specific membrane lectin operating as a cargo receptor for the transport of glycoproteins from the ER to the ERGIC. ERGIC-53 can also be used for studying aspects of protein trafficking in the secretory pathway.
Figure: Immunocytochemistry of HeLa cells using MAb to ERGIC-53 (G1/93) (Prod. No. ALX-804-602) at a 1:1'000 dilution.
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Figure 2: Immunocytochemistry of HeLa cells (fixed in methanol) using ERGIC-53, mAb (G1/93) (Prod. No. ALX-804-602) at a 1:2’000 dilution.
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Product Literature References

Coronavirus envelope (E) protein remains at the site of assembly: P. Venkatagopalan, et al.; Virology 478C, 75 (2015), Application(s): Immunofluorescence, Abstract;
Correction for Fernández de Castro et al., Reovirus Forms Neo-Organelles for Progeny Particle Assembly within Reorganized Cell Membranes: I. Fernández de Castro, et al.; MBio. 6, e02529-14 (2015), Application(s): Immunofluorescence Assay, Abstract; Full Text
Estrogen enhances secretion of apolipoprotein B-100 containing lipoproteins by BeWo cells: M. Kamper, et al.; Biochimie (2015), Application(s): Immunofluorescence, Abstract;
Neuroblastoma Tyrosine Kinase Signaling Networks Involve FYN and LYN in Endosomes and Lipid Rafts: J. Palacios-Moreno, et al.; PLoS Comput. Biol. 11, e1004130 (2015), Application(s): Fluorescent Imaging, Abstract; Full Text
The golgin GMAP-210 is required for efficient membrane trafficking in the early secretory pathway: P. Roboti, et al.; J. Cell. Sci. (2015), Application(s): Immunofluorescence Microscopy, Abstract; Full Text
Yip1A, a Novel Host Factor for the Activation of the IRE1 Pathway of the Unfolded Protein Response during Brucella Infection: Y. Taguchi, et al.; PLoS Pathog. 11, e1004747 (2015), Abstract; Full Text
Phosphatidylinositol- and phosphatidylcholine-transfer activity of PITPbeta is essential for COPI-mediated retrograde transport from the Golgi to the endoplasmic reticulum: N. Carvou, et al.; J. Cell Sci. 123, 1262 (2010), Abstract;
Selective export of human GPI-anchored proteins from the endoplasmic reticulum: C. Bonnon, et al.; J. Cell Sci. 123, 1705 (2010), Abstract;
Yip1A structures the mammalian endoplasmic reticulum: K.M. Dykstra, et al.; Mol. Biol. Cell 21, 1556 (2010), Abstract;
Intracellular phospholipase A1gamma (iPLA1gamma) is a novel factor involved in coat protein complex I- and Rab6-independent retrograde transport between the endoplasmic reticulum and the Golgi complex: R.K. Morikawa, et al.; J. Biol. Chem. 284, 26620 (2009), Abstract;
Deletion of 3 residues from the C-terminus of MCFD2 affects binding to ERGIC-53 and causes combined factor V and factor VIII deficiency: B. Nyfeler, et al.; Blood 111, 1299 (2008), Abstract;
The Cargo Receptors Surf4, Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)-53, and p25 Are Required to Maintain the Architecture of ERGIC and Golgi: S. Mitrovic, et al.; Mol. Biol. Cell 19, 1976 (2008), Abstract;
Bap31 enhances the endoplasmic reticulum export and quality control of human class I MHC molecules: J.J. Ladasky, et al.; J. Immunol. 177, 6172 (2006), Abstract;
Capturing protein interactions in the secretory pathway of living cells: B. Nyfeler, et al.; PNAS 102, 6350 (2005), Abstract; Full Text
pH-induced conversion of the transport lectin ERGIC-53 triggers glycoprotein release: C. Appenzeller-Herzog, et al.; J. Biol. Chem. 279, 12943 (2004), Abstract; Full Text
Proteomics of endoplasmic reticulum-golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46: L. Breuza, et al.; J. Biol. Chem. 279, 47242 (2004), Abstract; Full Text
Abnormal acidification of melanoma cells induces tyrosinase retention in the early secretory pathway: R. Halaban, et al.; J. Biol. Chem. 277, 14821 (2002), Abstract; Full Text
The recycling of ERGIC-53 in the early secretory pathway. ERGIC-53 carries a cytosolic endoplasmic reticulum-exit determinant interacting with COPII: F. Kappeler, et al.; J. Biol. Chem. 272, 31801 (1997), Abstract; Full Text
ERGIC-53 is a functional mannose-selective and calcium-dependent human homologue of leguminous lectins: C. Itin, et al.; Mol. Biol. Cell 7, 483 (1996), Abstract; Full Text
Targeting of protein ERGIC-53 to the ER/ERGIC/cis-Golgi recycling pathway: C. Itin, et al.; J. Cell Biol. 131, 57 (1995), Abstract; Full Text
A dual role for COOH-terminal lysine residues in pre-Golgi retention and endocytosis of ERGIC-53: F. Kappeler, et al.; J. Biol. Chem. 269, 6279 (1994), Abstract; Full Text
The isolated ER-Golgi intermediate compartment exhibits properties that are different from ER and cis-Golgi: A. Schweizer, et al.; J. Cell Biol. 113, 45 (1991), Abstract; Full Text
Identification, by a monoclonal antibody, of a 53-kD protein associated with a tubulo-vesicular compartment at the cis-side of the Golgi apparatus: A. Schweizer, et al.; J. Cell Biol. 107, 1643 (1988), Abstract; Full Text

General Literature References

ERGIC-53 and traffic in the secretory pathway: H.P. Hauri, et al.; J. Cell Sci. 113, 587 (2000), Abstract; Full Text

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