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Giantin monoclonal antibody (G1/133)

 
ALX-804-600-C100 100 µg 356.00 USD
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Product Specification

Alternative Name:Macrogolgin, Golgi complex-associated 372kDa protein, GCP372
 
Clone:G1/133
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Giantin from Caco-2 cell Golgi membrane fraction.
 
UniProt ID:Q14789
 
Species reactivity:Avian, Mammalian, Monkey
 
Applications:ELISA, Flow Cytometry, ICC, IHC (FS), IP, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (1:1,000)
Immunoprecipitation (2µl)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Not recommended for Western Blot
 
Purity Detail:Affinity purified (HYDRA column) and gel filtrated (FPLC).
 
Formulation:Lyophilized. Preservative free.
 
Reconstitution:Reconstitute with 100µl PBS containing 0.1% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Giantin, the largest Golgi membrane protein, is C-terminally anchored to cis- and medial-Golgi membranes and forms a long ~250nm coiled-coil rod that directly interacts with microtubules in a cell-cycle regulated way. It is an excellent marker for mammalian golgi complex. Giantin plays also a role in docking COPI vesicles to Golgi membranes. It is speculated that after mitosis giantin aids in guiding vesiculated Golgi membranes to the microtubule organizing center to reform a new Golgi apparatus.
 

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Product Literature References

Golgi membrane fission requires the CtBP1-S/BARS-induced activation of lysophosphatidic acid acyltransferase δ: A. Pagliuso, et al.; Nat. Commun. 7, 12148 (2016), Application(s): Antibody for immunofluorescence, Abstract; Full Text
Iterative sorting of apical and basolateral cargo in MDCK cells: A. Treyer, et al.; Mol. Biol. Cell (2016), Application(s): Immunofluorescence, Abstract; Full Text
Substrate Requirements for SPPL2b-dependent Regulated Intramembrane Proteolysis: L. Martin, et al.; J. Biol. Chem. 284, 5662 (2009), Abstract; Full Text
The Cargo Receptors Surf4, Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)-53, and p25 Are Required to Maintain the Architecture of ERGIC and Golgi: S. Mitrovic, et al.; Mol. Biol. Cell 19, 1976 (2008), Abstract;
Capturing protein interactions in the secretory pathway of living cells: B. Nyfeler, et al.; PNAS 102, 6350 (2005), Abstract; Full Text
Proteomics of endoplasmic reticulum-golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46: L. Breuza, et al.; J. Biol. Chem. 279, 47242 (2004), Abstract; Full Text
Mistargeting of the lectin ERGIC-53 to the endoplasmic reticulum of HeLa cells impairs the secretion of a lysosomal enzyme: F. Vollenweider, et al.; J. Cell Biol. 142, 377 (1998), Abstract; Full Text
Targeting of protein ERGIC-53 to the ER/ERGIC/cis-Golgi recycling pathway: C. Itin, et al.; J. Cell Biol. 131, 57 (1995), Abstract; Full Text
Giantin, a novel conserved Golgi membrane protein containing a cytoplasmic domain of at least 350 kDa: A.D. Linstedt and H.P. Hauri; Mol. Biol. Cell 4, 679 (1993), Abstract; Full Text

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