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Toll-like receptor 5 monoclonal antibody (19D759.2)

 
ALX-804-598-C100 100 µg 345.00 USD
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Product Specification

Alternative Name:TLR5, Toll/Interleukin-1 receptor-like gene 3, TIL3
 
Clone:19D759.2
 
Host:Mouse
 
Isotype:IgG2a
 
Immunogen:Synthetic peptide corresponding to a portion of aa 700-800 of human TLR5 (Toll-like receptor 5).
 
UniProt ID:O60602
 
Species reactivity:Human, Mouse
Dog
 
Specificity:Recognizes an intracellular epitope in the cytoplasmic domain of TLR5.
 
Applications:Flow Cytometry, IHC (FS), IHC (PS), WB
 
Recommended Dilutions/Conditions:Flow Cytometry (intracellular, 0.5-3µg/1 x 106 cells)
Immunohistochemistry (frozen sections, 10µg/ml)
Immunohistochemistry (paraffin sections, 5µg/ml)
Western Blot (1-3µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Flow Cytometry: Suitable for cell surface and intracellular FC.
 
Positive Control:Ramos cells can be used as positive control.
 
Purity Detail:Protein G-affinity purified.
 
Formulation:Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.
 
Use/Stability:Stable for 6 months when stored at +4°C.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
804-598 wb
Figure 1: Western blot analysis of TLR5 in A) Ramos and B) Raw cell lysate using MAb to Toll-like Receptor 5 (19D759.2) (Prod. No. ALX-804-598) at 2µg/ml.
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Figure 2: Intracellular flow analysis of TLR5 in Ramos cells using 1ug of MAb to Toll-like Receptor 5 (19D759.2) (Prod. No. ALX-804-598).  Shaded histogram represents cells without antibody; green represents isotype control (BD Biosciences); orange represents MAb to Toll-like Receptor 5 (19D759.2). Secondary antibody: Goat Anti-Mouse IgG PE.
804-598 fc new2
Figure 3:  Intracellular flow analysis of TLR5 in human PBMC (monocytes)  using 0.5 ug of MAb to Toll-like Receptor 5 (19D759.2) (Prod. No. ALX-804-598).  Shaded histogram represents cells without antibody; green represents isotype control (BD Biosciences); red represents MAb to Toll-like Receptor 5 (19D759.2). Secondary antibody: Goat Anti-Mouse IgG PE.
804-598 ihc-paraffin
Figure 4: Immunohistochemical analysis of TLR5 in formalin-fixed, paraffin-embedded mouse brain tissue using an isotype (top) and to MAb to Toll-like Receptor 5 (19D759.2) (Prod. No. ALX-804-598) (bottom) at 5μg/ml.
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Product Literature References

The myeloid differentiation factor 88 (MyD88) is required for CD4+ T cell effector function in a murine model of inflammatory bowel disease: M. Fukata, et al.; J. Immunol. 180, 1886 (2008), Abstract;
Human CD4+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4+CD25+ T regulatory cells: N.K. Crellin, et al.; J. Immunol. 175, 8051 (2005), Abstract;
Inhibition of neutrophil apoptosis by TLR agonists in whole blood: involvement of the phosphoinositide 3-kinase/Akt and NF-kappaB signaling pathways, leading to increased levels of Mcl-1, A1, and phosphorylated Bad: S. Francois, et al.; J. Immunol. 174, 3633 (2005), Abstract; Full Text
TGF-alpha regulates TLR expression and function on epidermal keratinocytes: L.S. Miller, et al.; J. Immunol. 174, 6137 (2005), Abstract; Full Text

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