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[pSer82]HSP27 monoclonal antibody (5B9)

ALX-804-588-C100 100 µg 374.00 USD
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Replaces Prod. #: BML-SA348

Product Specification

Alternative Name:Heat shock protein 27
Immunogen:Synthetic peptide corresponding to aa 79-85 (R79QLpSSGV85) of human HSP27 (heat shock protein 27) phosphorylated at Ser82 conjugated to KLH.
UniProt ID:P04792
GenBank ID:L39370
Species reactivity:Human, Mouse, Rat
Specificity:Recognizes HSP27 phosphorylated at Ser82.
Applications:IHC, WB
Recommended Dilutions/Conditions:Western Blot (0.5µg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Positive Control:Included. (Prod. No. ALX-840-031)
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
Use/Stability:Upon reconstitution, stable at -80°C for up to 1 year, at +4°C for up to 3 months.
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Technical Info/Product Notes:Replacement for ADI-905-642

Product Literature References

The mechanisms of the widespread production of phosphorylated HSP25 after fatiguing muscle stimulation: Y. Jammes, et al.; J. Exp. Biol. 216, 3620 (2013), Application(s): Use as detection antibody in ELISA, Abstract; Full Text

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