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SEL1L monoclonal antibody (MSel1)

 
ALX-804-579-C100 100 µg 378.00 USD
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Product Specification

Alternative Name:Suppressor of lin-12-like protein
 
Clone:MSel1
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant human SEL1L (suppressor of lin-12-like protein).
 
UniProt ID:Q9UBV2
 
Species reactivity:Human, Mouse
 
Applications:IHC (FS), IHC (PS), IP, WB
 
Recommended Dilutions/Conditions:Western Blot (10µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In 150mM sodium chloride, pH 7.4, containing 0.02% sodium azide.
 
Handling:Do not freeze.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
Scientific Background:SEL1L (suppressor of lin-12-like protein) is the human ortholog of the C. elegans sel-1 gene, a negative regulator of the lin-12/Notch family of proteins. Human SEL1L is a component of endoplasmic reticulum-associated degradation (ERAD), involved in ubiquitin-dependent degradation of misfolded ER proteins. In addition, SEL1L plays a role in cancer progression. Several studies provided significant evidence that increased SEL1L expression is linked to a decrease in tumor aggressiveness.
 
804-579
Figure: Western blot analysis using MAb to SEL1L (MSel1) (Prod. No. ALX-804-579).Lane 1: MAb to SEL1L (MSel1)Lane 2: Immunoprecipitation of total lysates from BT-474 cells followed by Western blot analysis using MAb to SEL1L (MSel1) (Prod. No. ALX-804-579) (see method below)Lane 3: No lysateLane 4: MW marker. Method: Cells were lysed using a buffer containing 50mM TRIS-HCl, pH 7.4, 150mM NaCl, 10% glycerol, 0.5% NP40 and protease inhibitors (10µg/ml aproteinin, 1µg/ml leupeptin, 1µg/ml PMSF) for 10 min. at 4°C. Lysates were then centrifuged at 12’000 rpm for 10 min. at 4°C to remove insoluble material. Protein concentration was determined by Comassie Plus Protein Assay (Pierce, Rockford, IL). Protein extracts (1.5mg/sample) were immunoprecipitated for 2 hours at 4°C using 5µg of SEL1L and 20µl of protein-A/G Sepharose beads (Pharmacia-Amersham). Precipitates were then separated on 10% SDS-PAGE, transferred onto nitrocellulose membrane (Amersham) and probed with 10µg/ml MAb to SEL1L (MSel1). Proteins were revealed using ECL-Plus chemiluminescence (Amersham) following the manufacturer’s instructions.Note: MAb to SEL1L (MSel1) can recognize isoforms of SEL1L protein with MW lower than 88kDa.
804-579 2
Figure 2: Pancreatic adenocarcinomas: (b) with and (c) without SEL1L expression.
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Product Literature References

A different pathway in the endoplasmic reticulum stress-induced expression of human HRD1 and SEL1 genes: M. Kaneko, et al.; FEBS Lett. 581, 5355 (2007), Abstract;
Simian Virus 40 depends on ER protein folding and quality control factors for entry into host cells: M. Schelhaas, et al.; Cell 131, 516 (2007), Abstract;
SEL1L a multifaceted protein playing a role in tumor progression: I. Biunno, et al.; J. Cell. Physiol. 208, 23 (2006), Abstract;
SEL1L expression in pancreatic adenocarcinoma parallels SMAD4 expression and delays tumor growth in vitro and in vivo: M. Cattaneo, et al.; Oncogene 22, 6359 (2003), Abstract;
Production of a monoclonal antibody directed against the recombinant SEL1L protein: R. Orlandi, et al.; J. Biol. Markers 17, 104 (2002), Abstract;
SEL1L expression decreases breast tumor cell aggressiveness in vivo and in vitro: R. Orlandi, et al.; Cancer Res. 62, 567 (2002), Abstract; Full Text

General Literature References

A different pathway in the endoplasmic reticulum stress-induced expression of human HRD1 and SEL1 genes: M. Kaneko, et al.; FEBS Lett. 581, 5355 (2007), Abstract;
SEL1L a multifaceted protein playing a role in tumor progression: I. Biunno, et al.; J. Cell Physiol. 208, 23 (2006), Abstract;
Complete cDNA sequence and genomic organization of a human pancreas-specific gene homologous to Caenorhabditis elegans sel-1: Y. Harada, et al.; J. Hum. Genet. 44, 330 (1999), Abstract;

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