Akt, mAb (5C10)



ALX-804-553-C100	100 µg	379.00 USD

Product Specification

Alternative Name:	Protein kinase B, PKB

Purity Detail:	Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.

Formulation:	Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.

Clone:	5C10

Isotype:	Mouse IgG1

Immunogen:	Synthetic peptide corresponding to C-terminal human Akt (protein kinase B; PKB).

Reconstitution:	Reconstitute with 1ml distilled water (15min, RT).

Specificity:	Recognizes a c-terminal epitope (aa 466-480) of phosphorylated and non-phosphorylated human, mouse, rat and dog Akt isoforms (see Technical Note below).

Application:	Western Blot (0.5µg/ml for HRPO/ECL detection). Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4'000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.

Long Term Storage:	-20°C

Use/Stability:	Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.

Handling:	After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.

Positive Control Included:	Included (Prod. No. ALX-840-030).

Background / Technical Information:	Technical Note: There are three mammalian isoforms of Akt: Akt1 (PKBa), Akt2 (PKBb), and Akt3 (PKBg). Akt1 shares 81% and 83% amino acid identity with Akt2 and Akt3, respectively. Although the three isoforms show broad tissue distribution, Akt1 is the most ubiquitously expressed. Akt2 is expressed at a lower level than Akt1 except in insulin-responsive tissues where it predominates. In particular, Akt2 is more abundant and more highly activated than Akt1 in adipocytes. Akt3 is expressed at the lowest level except in testes and brain. The C-terminal sequence of the three isoforms are shown below. Note that the phosphorylation site slightly differs between the three isoforms. Thus the phosphorylation site Ser⁴⁷³ of Akt1 corresponds to Ser⁴⁷⁴ in Akt2 and Ser⁴⁷² in Akt3 respectively. Akt1: V⁴⁶¹DSERRPHFPQFS⁴⁷³YSASSTA⁴⁸⁰ UniProt ID P31749 Akt2: L⁴⁶¹LELDQRTHFPQFS⁴⁷⁴YSASIRE⁴⁸¹ UniProt ID P31751 Akt3: D⁴⁶¹NERRPHFPQFS⁴⁷²YSASGRE⁴⁷⁹ UniProt IDQ9Y243 Replacement for ADI-905-661