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CHOP monoclonal antibody (9C8)

ALX-804-551-C100 100 µg 590.00 USD
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Product Specification

Alternative Name:C/EBP homology protein, Growth arrest and DNA damage inducible protein 153, GADD153
Immunogen:Recombinant mouse CHOP (C/EBP homology protein).
UniProt ID:P35639
Species reactivity:Human, Mouse
Specificity:Recognizes an N-terminal epitope.
Applications:ICC, IP, WB
Recommended Dilutions/Conditions:Western Blot (1µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~31kDa by Western blot.
Formulation:Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:CHOP is a small nuclear protein that is capable of dimerizing with transcription factors C/EBP α and β. Once dimerized, this complex inhibits the normal binding and function of C/EBP to classical binding sites. Inversely, the C/EBP-CHOP dimer gains binding activity to other non-classical C/EBP stress related targets.
Under normal cellular conditions this protein is not expressed in detectable levels, but is highly unregulated during times of cellular/ER stress. Examples of CHOP inducing stress include: treatment with tunicamycin, nutrient starvation and reducing agents that interfere with the calcium flux across the ER membrane.
Figure: Western blot of endogenous CHOP from primary human fibroblasts using 9C8 (Prod. No. ALX-804-551). Lane 1: Untreated cells. Lane 2: Cells treated with tunicamycin for 10 hours.
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Product Literature References

Cadmium induces Wnt signaling to upregulate proliferation and survival genes in sub-confluent kidney proximal tubule cells: P.K. Chakraborty, et al.; Mol Cancer 9, 102 (2010), Abstract;
Maximal killing of lymphoma cells by DNA damage-inducing therapy requires not only the p53 targets Puma and Noxa, but also Bim: L. Happo, et al.; Blood 116, 5256 (2010), Abstract;
Regulated increase in folding capacity prevents unfolded protein stress in the ER: C. Christis, et al.; J. Cell. Sci. 123, 787 (2010), Abstract;
Comparative proteomics profiling of a phospholamban mutant mouse model of dilated cardiomyopathy reveals progressive intracellular stress responses: A.O. Gramolini, et al.; Mol. Cell Proteomics 7, 519 (2008), Abstract;
CHOP-Dependent stress-inducible expression of a novel form of carbonic anhydrase VI: J. Sok, et al.; Mol. Cell. Biol. 19, 495 (1999), Abstract; Full Text
Induction of a secreted protein by the myxoid liposarcoma oncogene: M. Kuroda, et al.; PNAS 96, 5025 (1999), Abstract; Full Text
Cloning of mammalian Ire1 reveals diversity in the ER stress responses: X.Z. Wang, et al.; EMBO J. 17, 5708 (1998), Abstract; Full Text
Identification of novel stress-induced genes downstream of chop: X.Z. Wang, et al.; EMBO J. 17, 3619 (1998), Abstract; Full Text
Regulation of the pro-B-cell-specific enhancer of the Id1 gene involves the C/EBP family of proteins: S. Saisanit and X.H. Sun; Mol. Cell. Biol. 17, 844 (1997), Abstract; Full Text
Signals from the stressed endoplasmic reticulum induce C/EBP-homologous protein (CHOP/GADD153): X.Z. Wang, et al.; Mol. Cell. Biol. 16, 4273 (1996), Abstract; Full Text
Inhibition of adipogenesis by the stress-induced protein CHOP (Gadd153): N. Batchvarova, et al.; EMBO J. 14, 4654 (1995), Abstract;

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