Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

CHOP monoclonal antibody (9C8)

 
ALX-804-551-C100 100 µg 590.00 USD
Do you need bulk/larger quantities?
 

Product Specification

Alternative Name:C/EBP homology protein, Growth arrest and DNA damage inducible protein 153, GADD153
 
Clone:9C8
 
Host:Mouse
 
Isotype:IgG2b
 
Immunogen:Recombinant mouse CHOP (C/EBP homology protein).
 
UniProt ID:P35639
 
Species reactivity:Human, Mouse
 
Specificity:Recognizes an N-terminal epitope.
 
Applications:ICC, IP, WB
 
Recommended Dilutions/Conditions:Western Blot (1µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~31kDa by Western blot.
 
Formulation:Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:CHOP is a small nuclear protein that is capable of dimerizing with transcription factors C/EBP α and β. Once dimerized, this complex inhibits the normal binding and function of C/EBP to classical binding sites. Inversely, the C/EBP-CHOP dimer gains binding activity to other non-classical C/EBP stress related targets.
Under normal cellular conditions this protein is not expressed in detectable levels, but is highly unregulated during times of cellular/ER stress. Examples of CHOP inducing stress include: treatment with tunicamycin, nutrient starvation and reducing agents that interfere with the calcium flux across the ER membrane.
 
CHOP monoclonal antibody (9C8) Western blot
Figure: Western blot of endogenous CHOP from primary human fibroblasts using 9C8 (Prod. No. ALX-804-551). Lane 1: Untreated cells. Lane 2: Cells treated with tunicamycin for 10 hours."
Please mouse over
CHOP monoclonal antibody (9C8) Western blot

Product Literature References

Cadmium induces Wnt signaling to upregulate proliferation and survival genes in sub-confluent kidney proximal tubule cells: P.K. Chakraborty, et al.; Mol Cancer 9, 102 (2010), Abstract;
Maximal killing of lymphoma cells by DNA damage-inducing therapy requires not only the p53 targets Puma and Noxa, but also Bim: L. Happo, et al.; Blood 116, 5256 (2010), Abstract;
Regulated increase in folding capacity prevents unfolded protein stress in the ER: C. Christis, et al.; J. Cell. Sci. 123, 787 (2010), Abstract;
Comparative proteomics profiling of a phospholamban mutant mouse model of dilated cardiomyopathy reveals progressive intracellular stress responses: A.O. Gramolini, et al.; Mol. Cell Proteomics 7, 519 (2008), Abstract;
CHOP-Dependent stress-inducible expression of a novel form of carbonic anhydrase VI: J. Sok, et al.; Mol. Cell. Biol. 19, 495 (1999), Abstract; Full Text
Induction of a secreted protein by the myxoid liposarcoma oncogene: M. Kuroda, et al.; PNAS 96, 5025 (1999), Abstract; Full Text
Cloning of mammalian Ire1 reveals diversity in the ER stress responses: X.Z. Wang, et al.; EMBO J. 17, 5708 (1998), Abstract; Full Text
Identification of novel stress-induced genes downstream of chop: X.Z. Wang, et al.; EMBO J. 17, 3619 (1998), Abstract; Full Text
Regulation of the pro-B-cell-specific enhancer of the Id1 gene involves the C/EBP family of proteins: S. Saisanit and X.H. Sun; Mol. Cell. Biol. 17, 844 (1997), Abstract; Full Text
Signals from the stressed endoplasmic reticulum induce C/EBP-homologous protein (CHOP/GADD153): X.Z. Wang, et al.; Mol. Cell. Biol. 16, 4273 (1996), Abstract; Full Text
Inhibition of adipogenesis by the stress-induced protein CHOP (Gadd153): N. Batchvarova, et al.; EMBO J. 14, 4654 (1995), Abstract;

Related Literature

Brochures
Comprehensive Tools for Quantifying Cellular Responses to Oxidative Damage
Comprehensive Tools for Quantifying Cellular Responses to Oxidative Damage
Download as PDF

Technical Posters
Disease-Associated Stress Signaling
Disease-Associated Stress Signaling
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2019 Enzo Life Sciences, Inc.,