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Caspase-1 (mouse) monoclonal antibody (4G8)

 
ALX-804-531-C100 100 µg 334.00 USD
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Product Specification

Alternative Name:IL-1β converting enzyme, Interleukin-1β converting enzyme, ICE
 
Clone:4G8
 
Host:Rat
 
Isotype:IgG1
 
Immunogen:p20 fragment of mouse caspase-1 and synthetic peptide corresponding to aa 206-220 (T206ALEMVKEVKEFAAC220) of mouse caspase-1.
 
UniProt ID:P29452
 
Species reactivity:Mouse
 
Specificity:Recognizes an epitope within the p20 fragment of caspase-1.
 
Crossreactivity:Does not cross-react with mouse caspase-11 or -12.
 
Applications:ELISA, Flow Cytometry, WB
 
Application Notes:Detects a band of ~45kDa by Western blot.
 
Formulation:Liquid. Protein G Sepharose™ purified antibody in PBS containing 0.02% sodium azide.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:Capases are a highly conserved family of cysteine proteases and are synthesized as inactive zymogens, which are cleaved at Asp-x sites during apoptosis, generating large and small subunits that heterodimerize to form the active enzyme. Caspase-1 is a member of the ICE/CED-3 family of caspases and is detected in the pro-form as a protein of 45 kDa.
Caspase-1 generates the bioactive form of the pro-inflammatory cytokine IL-1β from its biologically inactive precursor. Overexpression of caspase-1 in cells induces apoptosis, which can be inhibited by cytokine response modifier A (CrmA). Caspase-1 mRNA is predominantly expressed in the spleen and lung and to a lesser extent in skeletal muscle, kidney, testis and heart and within these tissues macrophages preferentially express caspase-1. Caspase-1 deficient mice have no gross abnormalities, however, macrophages from caspase-1-deficient mice are defective in IL-β processing and release.
 
Technical Info/Product Notes:SEPHAROSE is a trademark of GE Healthcare companies.
 
804-531
Figure A: MAbs to caspase-1 (clone 1H11 (Prod.No. ALX-804-530) and clone 4G8 (Prod. No. ALX-804-531)) detect overexpressed caspase-1 in 293T cells transiently transfected with an EE tagged inactive cysteine mutant of mouse caspase-1 by flow cytometry. Figure B: MAbs to caspase-1 (clone 1H11 (Prod.No. ALX-804-530) and clone 4G8 (Prod. No. ALX-804-531)) detect overexpressed caspase-1 in 293T cells transiently transfected with a EE tagged inactive cysteine mutant of mouse caspase-1 as a band of ~45 kDa.
Figure C: MAbs to caspase-1 (clone 1H11 (Prod.No. ALX-804-530) and clone 4G8 (Prod. No. ALX-804-531)) detect endogenous pro-caspase-1 in spleen from WT mice, but not from spleen of caspase-1 deficient mice. HSP-70: loading control.
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804-531

Product Literature References

Amyloid-beta oligomerization is associated with the generation of a typical peptide fragment fingerprint: N. Rudinskiy, et al.; Alzheimers Dement. 12, 996 (2016), Application(s): SDS-PAGE and immunoblotting, Abstract;

General Literature References

Caspase-2 is not required for thymocyte or neuronal apoptosis even though cleavage of caspase-2 is dependent on both Apaf-1 and caspase-9: L.A. O'Reilly, et al.; Cell Death Differ. 9, 832 (2002), Methodology, Abstract;
Rapid hybridoma screening method for the identification of monoclonal antibodies to low-abundance cytoplasmic proteins: L.A. O'Reilly, et al.; Biotechniques 25, 824 (1998), Methodology, Abstract;

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For Research Use Only. Not for use in diagnostic procedures.
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