Product Details
Alternative Name: | IL-1β converting enzyme, Interleukin-1β converting enzyme, ICE |
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Clone: | 1H11 |
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Host: | Rat |
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Isotype: | IgG1 |
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Immunogen: | p20 fragment of mouse caspase-1 and synthetic peptide corresponding to aa 206-220 (T206ALEMVKEVKEFAAC220) of mouse caspase-1. |
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UniProt ID: | P29452 |
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Species reactivity: | Mouse
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Specificity: | Recognizes an epitope within the p20 fragment of caspase-1. |
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Crossreactivity: | Does not cross-react with mouse caspase-11 or -12. |
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Applications: | ELISA, Flow Cytometry, WB
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Application Notes: | Detects a band of ~45kDa by Western blot. |
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Formulation: | Liquid. Protein G Sepharose™ purified antibody in PBS containing 0.02% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Short Term Storage: | +4°C |
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Long Term Storage: | -20°C |
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Scientific Background: | Capases are a highly conserved family of cysteine proteases and are synthesized as inactive zymogens, which are cleaved at Asp-x sites during apoptosis, generating large and small subunits that heterodimerize to form the active enzyme. Caspase-1 is a member of the ICE/CED-3 family of caspases and is detected in the pro-form as a protein of 45 kDa. Caspase-1 generates the bioactive form of the pro-inflammatory cytokine IL-1β from its biologically inactive precursor. Overexpression of caspase-1 in cells induces apoptosis, which can be inhibited by cytokine response modifier A (CrmA). Caspase-1 mRNA is predominantly expressed in the spleen and lung and to a lesser extent in skeletal muscle, kidney, testis and heart and within these tissues macrophages preferentially express caspase-1. Caspase-1 deficient mice have no gross abnormalities, however, macrophages from caspase-1-deficient mice are defective in IL-β processing and release. |
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Technical Info/Product Notes: | SEPHAROSE is a trademark of GE Healthcare companies. |
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Regulatory Status: | RUO - Research Use Only |
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Figure A: MAbs to caspase-1 (clone 1H11 (
Prod.No. ALX-804-530) and clone 4G8 (
Prod. No. ALX-804-531)) detect overexpressed caspase-1 in 293T cells transiently transfected with an EE tagged inactive cysteine mutant of mouse caspase-1 by flow cytometry.
Figure B: MAbs to caspase-1 (clone 1H11 (
Prod.No. ALX-804-530) and clone 4G8 (
Prod. No. ALX-804-531)) detect overexpressed caspase-1 in 293T cells transiently transfected with a EE tagged inactive cysteine mutant of mouse caspase-1 as a band of ~45 kDa.
Figure C: MAbs to caspase-1 (clone 1H11 (
Prod.No. ALX-804-530) and clone 4G8 (
Prod. No. ALX-804-531)) detect endogenous pro-caspase-1 in spleen from WT mice, but not from spleen of caspase-1 deficient mice. HSP-70: loading control."
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Product Literature References
Apoptosis and non-inflammatory phagocytosis can be induced by mitochondrial damage without caspases: M.F. Van Delft, et al.; Cell Death Differ.
17, 821 (2010),
Application(s): WB using mouse thymocytes cell lysate,
Abstract;
Full Text
Apaf-1 and caspase-9 are required for cytokine withdrawal-induced apoptosis of mast cells but dispensable for their functional and clonogenic death: V.S. Marsden, et al.; Blood
107, 1872 (2006),
Abstract;
Full Text
General Literature References
Caspase-2 is not required for thymocyte or neuronal apoptosis even though cleavage of caspase-2 is dependent on both Apaf-1 and caspase-9: L.A. O'Reilly, et al.; Cell Death Differ.
9, 832 (2002), Methodology,
Abstract;
Rapid hybridoma screening method for the identification of monoclonal antibodies to low-abundance cytoplasmic proteins: L.A. O'Reilly, et al.; Biotechniques
25, 824 (1998), Methodology,
Abstract;