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BimS/EL/L monoclonal antibody (10B12)

 
ALX-804-528-C100 100 µg 356.00 USD
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Product Specification

Alternative Name:BCL2L11
 
Clone:10B12
 
Host:Rat
 
Isotype:IgG2a
 
Immunogen:Synthetic peptide corresponding to aa 20-40 (Q20PAERPPQLRPGAPTSLQTEP40) of mouse BimL.
 
UniProt ID:O54918
 
Species reactivity:Human, Mouse, Rat
Dog, Monkey
 
Specificity:Recognizes an epitope within aa 20-40 of BimL, BimEL, and BimS. Also recognizes the six novel isoforms of human Bim, generated by alternative splicing and lacking the C-terminal hydrophobic region recently identified.
 
Applications:ELISA, Flow Cytometry, ICC, IHC, IP, WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (only EM)
Immunoprecipitation (2µg/ml)
Western Blot (1-5µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Formulation:Liquid. Protein G affinity purified antibody in PBS containing 25% glycerol and 0.02% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
804-528
Figure A: Hybridomas producing MAbs to Bim (peptide) were identified using clones of the IL-3-dependent mouse myeloid cell line FDC-P1 stably expressing Bcl-2 alone, or Bcl-2 plus mouse BimL mixed at a 1:1 ratio. Transfected cells were fixed, permeabilized and stained with the rat anti-Bim MAb (clone 10B12) (Prod. No. ALX-804-528), the mouse anti-Glu-Glu (EE) MAb (positive control) or with secondary antibody alone (negative control). Staining was visualised by FITC-conjugated goat anti-rat or anti-mouse IgG antibodies.
Figure B: Western blotting with anti-Bim MAb (clone 10B12) (Prod. No. ALX-804-528) recognizes all known isforms of Bim. Western blotting of lysates of 293T cells cells transfected with EE-tagged forms of mouse or HA tagged forms of human Bim. Blots were probed with anti-Bim MAb (clone 10B12) (Prod. No. ALX-804-528), which detected EE mouse BimS, BimL, BimEL and EE human, BimSS, BimS, BimLS, BimL, BimELS, BimEL. Probing with an anti-Hsp-70 MAb was used as a loading control for all blots.
Figure C: Anti-Bim MAb (clone 10B12) (Prod. No. ALX-804-528) detects endogenous Bim expression in mouse tissues but not those from Bim knockout mice. Bim protein was revealed in lysates from mouse tissues (5 x 106/lane) by Western blotting with anti-Bim MAb (clone 10B12) (Prod. No. ALX-804-528) and detection by ECL. Probing with an anti-Hsp-70 MAb was used as a loading control.
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Product Literature References

MEK/ERK-Mediated Phosphorylation of Bim is Required to Ensure Survival of T and B Lymphocytes during Mitogenic Stimulation.: L.A. O'Reilly, et al.; J. Immunol. 183, 261 (2009), Abstract;
Loss of the BH3-only protein Bmf impairs B cell homeostasis and accelerates gamma irradiation-induced thymic lymphoma development: V. Labi, et al.; J. Exp. Med. 205, 641 (2008), Abstract; Full Text
Generalised resistance to thymic deletion in the NOD mouse: a polygenic trait characterized by defective induction of Bim.: A. Liston, et al.; Immunity 21, 817 (2004), Abstract;

General Literature References

Identification of novel isoforms of the BH3 domain protein Bim which directly activate Bax to trigger apoptosis: Marani et al.; Mol. Cell. Biol. 22, 3577 (2002), Abstract; Full Text
Molecular cloning and characterization of six novel isoforms of human Bim, a member of the proapoptotic Bcl-2 family: U et al.; FEBS Lett. 509, 135 (2001), Abstract;
The proapoptotic BH3-only protein bim is expressed in hematopoietic, epithelial, neuronal, and germ cells: L.A. O'Reilly, et al.; Am. J. Pathol. 157, 449 (2000), Abstract; Full Text
Proapoptotic Bcl-2 relative Bim required for certain apoptotic responses, leukocyte homeostasis, and to preclude autoimmunity: P. Bouillet, et al.; Science 286, 1735 (1999), Abstract;
The proapoptotic activity of the Bcl-2 family member Bim is regulated by interaction with the dynein motor complex: H. Puthalakath, et al.; Mol. Cell 3, 287 (1999), Abstract;
A rapid hybridoma screening method for the identification of monoclonal antibodies to low-abundance cytoplasmic proteins: L.A. O'Reilly, et al.; Biotechniques 25, 824 (1998), Abstract;

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