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BimS/EL/L monoclonal antibody (3C5)

 
ALX-804-527-C100 100 µg 356.00 USD
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Product Specification

Alternative Name:BCL2L11
 
Clone:3C5
 
Host:Rat
 
Isotype:IgG2a
 
Immunogen:Synthetic peptide corresponding to aa 20-40 (Q20PAERPPQLRPGAPTSLQTEP40) of mouse BimL.
 
UniProt ID:O54918
 
Species reactivity:Human, Mouse, Rat
Dog, Monkey
 
Specificity:Recognizes an epitope within aa 20-40 of imL (~19kDa), BimEL (~23kDa), and BimS (~16kDa). Also recognizes the six novel isoforms of human Bim, generated by alternative splicing and lacking the C-terminal hydrophobic region recently identified.
 
Applications:ELISA, Flow Cytometry, ICC, IP, WB
 
Recommended Dilutions/Conditions:Immunoprecipitation (2µg/ml)
Western Blot (1-5µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein G-affinity purified.
 
Formulation:Liquid. In PBS containing 0.02% sodium azide and 25% glycerol.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
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Figure A: Hybridomas producing MAbs to Bim (peptide) were identified using clones of the IL-3-dependent mouse myeloid cell line FDC-P1 stably expressing Bcl-2 alone, or Bcl-2 plus mouse BimL mixed at a 1:1 ratio. Transfected cells were fixed, permeabilized and stained with the rat anti-Bim MAb (clone 3C5) (Prod. No. ALX-804-527), the mouse anti-Glu-Glu (EE) MAb (positive control) or with secondary antibody alone (negative control). Staining was visualised by FITC-conjugated goat anti-rat or anti-mouse IgG antibodies.Figure B: Western blotting with anti-Bim MAb (clone 3C5) (Prod. No. ALX-804-527) recognizes all known isoforms of Bim. Western blotting of lysates of 293T cells cells transfected with EE-tagged forms of mouse or HA tagged forms of human Bim. Blots were probed with anti-Bim MAb (clone 3C5) (Prod. No. ALX-804-527), which detected EE mouse BimS, BimL, BimEL and EE human, BimSS, BimS, BimLS, BimL, BimELS, BimEL. Probing with an anti-Hsp-70 MAb was used as a loading control for all blots.Figure C: Anti-Bim MAb (clone 3C5) (Prod. No. ALX-804-527) detects endogenous Bim expression in mouse tissues but not those from Bim knockout mice. Bim protein was revealed in lysates from mouse tissues (5 x 106/lane) by Western blotting with anti-Bim MAb (clone 3C5) (Prod. No. ALX-804-527) and detection by ECL. Probing with an anti-Hsp-70 MAb was used as a loading control.
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Product Literature References

Chemical chaperone TUDCA prevents apoptosis and improves survival during polymicrobial sepsis in mice: M. Doerflinger, et al.; Sci. Rep. 6, 34702 (2016), Application(s): Mouse cells, Abstract; Full Text
MYC selects against reduced BCL2A1/A1 protein expression during B cell lymphomagenesis: M. Sochalska, et al.; Oncogene (2016), Application(s): Immunoblotting, Abstract;
The BH3-only protein BIM contributes to late-stage involution in the mouse mammary gland: F. Schuler, et al.; Cell Death Differ. 23, 41 (2016), Application(s): Immunofluorescence microscopy, Abstract; Full Text
Maximal killing of lymphoma cells by DNA damage-inducing therapy requires not only the p53 targets Puma and Noxa, but also Bim: L. Happo, et al.; Blood 116, 5256 (2010), Abstract;
MEK/ERK-Mediated Phosphorylation of Bim is Required to Ensure Survival of T and B Lymphocytes during Mitogenic Stimulation.: L.A. O'Reilly, et al.; J. Immunol. 183, 261 (2009), Abstract;
The role of BH3-only protein Bim extends beyond inhibiting Bcl-2–like prosurvival proteins.: D. Mérino, et al.; J. Cell. Biol. 186, 355 (2009), Abstract;
Loss of the BH3-only protein Bmf impairs B cell homeostasis and accelerates gamma irradiation-induced thymic lymphoma development: V. Labi, et al.; J. Exp. Med. 205, 641 (2008), Abstract; Full Text
NF-kappaB1 and c-Rel cooperate to promote the survival of TLR4-activated B cells by neutralizing Bim via distinct mechanisms.: A Banerjee, et al.; Immunobiology 112, 5063 (2008), Abstract;
Apoptosis-based dual molecular targeting by INNO-406, a second generation Bcr-Abl inhibitor and ABT-737, an inhibitor of anti-apoptotic Bcl-2 proteins against Bcr-Abl-positive leukemia.: J. Kuroda, et al; Cell Death Differ. 14, 1667 (2007), Abstract; Full Text
ER stress triggers apoptosis by activating BH-3 only protein Bim.: H. Puthalakuth, et al.; Cell 129, 1337 (2007), Abstract;
Bim and Bad mediate imatinib-induced killing of Bcr/Abl+ leukemic cells, and resistance due to their loss is overcome by a BH3 mimetic: J. Kuroda, et al.; PNAS 103, 14907 (2006), Abstract; Full Text
Generalised resistance to thymic deletion in the NOD mouse: a polygenic trait characterized by defective induction of Bim.: A. Liston, et al.; Immunity 21, 817 (2004), Abstract;

General Literature References

Identification of novel isoforms of the BH3 domain protein Bim which directly activate Bax to trigger apoptosis: Marani et al.; Mol. Cell. Biol. 22, 3577 (2002), Abstract; Full Text
Molecular cloning and characterization of six novel isoforms of human Bim, a member of the proapoptotic Bcl-2 family: U et al.; FEBS Lett. 509, 135 (2001), Abstract;
The proapoptotic BH3-only protein bim is expressed in hematopoietic, epithelial, neuronal, and germ cells: L.A. O'Reilly, et al.; Am. J. Pathol. 157, 449 (2000), Abstract; Full Text
Proapoptotic Bcl-2 relative Bim required for certain apoptotic responses, leukocyte homeostasis, and to preclude autoimmunity: P. Bouillet, et al.; Science 286, 1735 (1999), Abstract;
The proapoptotic activity of the Bcl-2 family member Bim is regulated by interaction with the dynein motor complex: H. Puthalakath, et al.; Mol. Cell 3, 287 (1999), Abstract;
A rapid hybridoma screening method for the identification of monoclonal antibodies to low-abundance cytoplasmic proteins: L.A. O'Reilly, et al.; Biotechniques 25, 824 (1998), Abstract;

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