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GITRL (mouse) monoclonal antibody (MGTL 10)

 
ALX-804-518-C050 50 µg 240.00 USD
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Product Specification

Alternative Name:AITRL, TNFSF 18, Glucocorticoid-induced TNF receptor ligand, Activation-inducible TNF-related ligand
 
Clone:MGTL 10
 
Host:Rat
 
Isotype:IgG2a
 
Immunogen:Recombinant mouse GITRL.
 
UniProt ID:Q7TS55
 
Species reactivity:Mouse
 
Specificity:Recognizes both natural and recombinant GITRL.
 
Crossreactivity:Does not cross-react with human GITRL.
 
Applications:ELISA, Flow Cytometry, WB
 
Recommended Dilutions/Conditions:ELISA (direct and indirect, 1:5,000-1:10,000)
Flow Cytometry (1:1,000)
Western Blot (1:2,000-1:5,000 using ECL; suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk; incubate 1 hour at room temperature)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Excellent for Flow Cytometry.
 
Formulation:Liquid. In PBS, pH 7.4. Contains no preservatives.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
804-518 2
Figure: RAW264.7 cells (a mouse macrophage cell line, expressing GITRL) were stained significantly with MGTL 10 (Prod No. ALX-804-518).
804-518
Figure: Mouse GITRL transfected HEK293 cells were stained significantly with MGTL 10 (Prod No. ALX-804-518). No binding was detected on empty vector transfected cells.
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Product Literature References

Soluble glucocorticoid-induced tumor necrosis factor receptor (sGITR) stimulates osteoclast differentiation in response to receptor activator of NF-kappaB ligand (RANKL) in osteoclast cells: H.H. Shin, et al.; Bone 36, 832 (2005), Abstract;

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