Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Caspase-12 (mouse) monoclonal antibody (12G6)

 
ALX-804-511-C100 100 µg 230.00 USD
Do you need bulk/larger quantities?
 

Product Specification

Clone:12G6
 
Host:Rat
 
Isotype:IgG1
 
Immunogen:p20 fragment of mouse caspase-12 and synthetic peptide corresponding to aa 183-205 (N183KKFDYLFDRDNADTDILNMQEL205) of mouse caspase-12.
 
UniProt ID:O08736
 
Species reactivity:Mouse
 
Specificity:Recognizes an epitope within the p20 fragment of caspase-12.
 
Crossreactivity:Does not cross-react with mouse caspase-1 or -11.
 
Applications:ELISA, Flow Cytometry, WB
 
Application Notes:Detects a band of ~51kDa by Western blot.
 
Formulation:Liquid. Protein G affinity purified antibody in PBS containing 0.02% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:Caspase-12 is a member of the ICE/CED-3 family of caspases and is detected as a protein of ~51kDa. Overexpression of caspase-12 in cells induces apoptosis. Capase-12 is localised to the endoplasmatic reticulum (ER) and activated by ER stress and specifically cleaves and activates pro-caspase-9 and is also thought to be involved in the apoptosis induced by viral infection. Caspase-12-deficient mice are resistant to ER stress-induced apoptosis. Caspase-12 is probably expressed in many and perhaps all mouse tissues; and is expressed at high levels in skeletal muscle, heart, lung and kidney, and at moderate levels in brain and liver. In many humans caspase-12 is a pseudo gene and not expressed.
 
804-509 a
Figure: Detection of overexpressed caspase-12 in 293T cells transiently transfected with a FLAG tagged inactive cysteine mutant of mouse caspase-12 as a band of ~51 kDa by Western blot using MAbs to caspase-12 (clones 11F10 and 12G6) (Prod. No. ALX-804-509 and ALX-804-511).
804-509 b
Figure: Detection of pro-caspase-12 in MEFs from mice by Western blot using MAbs to caspase-12 (clones 11F10 and 12G6) (Prod. No. ALX-804-509 and ALX-804-511). 
804-511
Figure: Detection of endogenous caspase-12 in mouse lung, liver, spleen and pancreas by Western blot using MAb to caspase-12 (12G6) (Prod. No. ALX-804-511) as a band of ~51kDa.  
Please mouse over
804-509 a 804-509 b 804-511

Product Toolbox

PRODUCT RESOURCES

Print as PDF
MSDS
Certificate of Analysis

RELATED PRODUCTS

By target:
Caspase
Caspase-12
By biological activity:
Caspase Monoclonal antibody
Caspase-12 Monoclonal antibody
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Recommend this page

 
Keep in touch

©2017 Enzo Life Sciences, Inc.,