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Bmf (mouse/rat) monoclonal antibody (17A9)

 
ALX-804-508-C100 100 µg 367.00 USD
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Product Specification

Alternative Name:Bcl-2-modifying factor
 
Clone:17A9
 
Host:Rat
 
Isotype:IgG2a
 
Immunogen:Recombinant full length mouse Bmf and synthetic peptides corresponding to aa 13-27 (D13VFQSEDGEPGTQPG27) and aa 112-126 (A112GSPLGEQPPEGQFL126) of mouse Bmf.
 
UniProt ID:Q91ZE9
 
Species reactivity:Mouse, Rat
 
Crossreactivity:Does not cross-react with human Bmf.
 
Applications:ELISA, Flow Cytometry, IP, WB
 
Recommended Dilutions/Conditions:Western Blot (1-2µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects bands of ~25kDa and ~30kDa by Western blot, corresponding to endogenous Bmf and BmfL.
 
Formulation:Liquid. Protein G affinity purified antibody in PBS containing 0.02% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
804-508 a
Figure: A) Detection of overexpressed Bmf in 293T cells transiently transfected with mouse Bmf  by Western blot using MAb to Bmf (17A9) (Prod. No. ALX-804-508).
B) Detection of endogenous Bmf by Western blot using MAb to Bmf (17A9) (Prod. No. ALX-804-508) in mouse spleen, lymph node and thymus as two bands of ~25 and ~30kDa, but not in these tissues derived from two knock-out Bmf strains del 372 and del 376.
804-508 b
Figure: C) Detection of mouse and rat Bmf by Western blot using MAb to Bmf (17A9) (Prod. No. ALX-804-508).
D) Immunoprecipitation of endogenous Bmf from mouse spleen cells using MAb to Bmf (17A9) (Prod. No. ALX-804-508).
804-508 c
Figure: Flow cytometry data of 293T cells transiently transfected with EE-tagged Bmf, fixed with 1% PFA, permeabilised with saponin and stained intracellularly with Bmf (mouse/rat), mAb (17A9) (Prod. No. ALX-804-508) or positive control anti-EE.
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Product Literature References

BH3-only protein Bmf mediates apoptosis upon inhibition of CAP-dependent protein synthesis: F. Grespi, et al.; Cell Death Differ. 17, 1672 (2010), Abstract; Full Text
BID, BIM, and PUMA are essential for activation of the BAX- and BAK-dependent cell death program: D. Ren, et al.; Science 330, 1390 (2010), Abstract; Full Text
The role of BH3-only protein Bim extends beyond inhibiting Bcl-2–like prosurvival proteins.: D. Mérino, et al.; J. Cell. Biol. 186, 355 (2009), Abstract;
Loss of the BH3-only protein Bmf impairs B cell homeostasis and accelerates gamma irradiation-induced thymic lymphoma development: V. Labi, et al.; J. Exp. Med. 205, 641 (2008), Abstract; Full Text
Broad degradation of proapoptotic proteins with the conserved Bcl-2 homology domain 3 during infection with Chlamydia trachomatis: S. Ying, et al.; Infect. Immun. 73, 1399 (2005), Abstract; Full Text

General Literature References

Bmf: a proapoptotic BH3-only protein regulated by interaction with the myosin V actin motor complex, activated by anoikis: H. Puthalakath, et al.; Science 293, 1829 (2001), Methodology, Abstract;
Rapid hybridoma screening method for the identification of monoclonal antibodies to low-abundance cytoplasmic proteins: L.A. O'Reilly, et al.; Biotechniques 25, 824 (1998), Methodology, Abstract;

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