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3-Nitrotyrosine monoclonal antibody (39B6)

 
ALX-804-505-C050 50 µg 134.00 USD
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Product Specification

Alternative Name:3-Ntyr
 
Clone:39B6
 
Host:Mouse
 
Isotype:IgG2a
 
Immunogen:3-(4-Hydroxy-3-nitrophenylacetamido) propionic acid-BSA conjugate.
 
Species reactivity:Species independent
 
Specificity:Recognizes 3-nitrotyrosine moieties.
 
Applications:ELISA, IHC (FS), IHC (PS), IP, WB
 
Recommended Dilutions/Conditions:ELISA (with antigen coated plates)
Western Blot (1:5,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Formulation:Liquid. In PBS containing 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
804-505
Lane 1: 0.5µg BSA.Lane 2: 0.1µg Nitrotyrosine-modified BSA (ALX-840-014).Clone 39B6 was used at a concentration of 1:5’000, secondary antibody (HRP-conjugated anti-mouse IgG): 1:5’000.
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804-505

Product Literature References

An Abnormal Nitric Oxide Metabolism Contributes to Brain Oxidative Stress in the Mouse Model for the Fragile X Syndrome, a Possible Role in Intellectual Disability: E. Lima-Cabello, et al.; Oxid. Med. Cell. Longev. 2016, Article ID 8548910 (2016), Application(s): Western blot, Abstract; Full Text
Adverse effects of the classic antioxidant uric acid in adipocytes: NADPH oxidase-mediated oxidative/nitrosative stress: Y.Y. Sautin, et al.; Am. J. Physiol. Cell Physiol. 293, C584 (2007), Abstract; Full Text
Comparison of nitrotyrosine antibodies and development of immunoassays for the detection of nitrated proteins: T. Franze, et al.; Analyst 129, 589 (2004), Abstract;
Inducible Nitric Oxide Synthase and Nitrotyrosine in Listeric Encephalitis: A Cross-species Study in Ruminants: H. Pfister, et al.; Vet. Pathology 39, 190 (2002), Abstract; Full Text
Immunodetection of 3-nitrotyrosine in the liver of zymosan-treated rats with a new monoclonal antibody: comparison to analysis by HPLC: I. Girault, et al.; Free Radic. Biol. Med. 31, 1375 (2001), Abstract;

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