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CD14 monoclonal antibody (biG 14)

 
ALX-804-497-C100 100 µg 258.00 USD
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Product Specification

Clone:biG 14
 
Host:Mouse
 
Isotype:IgG2a
 
Immunogen:Monocytes of different species and immunoaffinity purified soluble human CD14.
 
UniProt ID:P08571
 
Species reactivity:Human
Bovine, Dog, Guinea pig, Horse, Porcine
 
Specificity:Recognized epitope in human: aa 39-44.
 
Applications:ELISA, Flow Cytometry, WB, FUNC
 
Application Notes:Functional Application: inhibits binding of LPS to CD14; 1µg/ml MAb inhibits binding of 0.5µg/ml LPS to CD14.
 
Purity Detail:Protein G-affinity purified.
 
Formulation:Lyophilized from PBS. Contains no preservatives.
 
Reconstitution:Reconstitute with 100µl distilled water.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The CD14 glycoprotein, gp 55, is present on most monocytic and macrophage like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for endotoxin.
 
Technical Info/Product Notes:Binding titer at human CD14 transfected CHO-cells is more than 10'000.
 

Product Literature References

Mutation of amino acids 39-44 of human CD14 abrogates binding of lipopolysaccharide and Escherichia coli: F. Stelter, et al.; Eur J Biochem 243, 100 (1997), Abstract;

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