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PTX3 monoclonal antibody (MNB4)

 
ALX-804-464-C100 100 µg 452.00 USD
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Product Specification

Alternative Name:Pentraxin 3, TSG-14, TNF stimulated gene-14
 
Clone:MNB4
 
Host:Rat
 
Isotype:IgG2a
 
Immunogen:Recombinant human PTX3 (pentraxin 3).
 
UniProt ID:P26022
 
Species reactivity:Human, Rat
 
Specificity:Recognizes the N-terminus of PTX3.
 
Applications:ELISA, Flow Cytometry, IHC (FS), IP, WB
 
Recommended Dilutions/Conditions:ELISA (capture)
Immunohistochemistry (frozen sections, 1µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Excellent for ELISA (capture).
 
Formulation:Liquid. In PBS containing 0.1% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Protocol:Sandwich ELISA for PTX3
  1. 96 well-ELISA plates (Nunc MaxiSorp 446612) were coated with 100µl of MAb to PTX3 (human) (MNB4) (Prod. No. ALX-804-464), 1μg/ml in 100µl coating buffer (15mM carbonate buffer pH 9.6) and incubated overnight at 4°C.
  2. After incubation, the plates were washed three times with 300µl/well of washing buffer (PBS + 0.05% Tween 20), and then 300µl of 5% dry milk in washing buffer were added to block non-specific binding.
  3. The plates were incubated for 2 hours at room temperature and then washed three times with washing buffer.
  4. 50µl (in duplicate) recombinant human PTX3 standard (Prod. No. ALX-201-124) (75pg/ml to 2.4ng/ml ), cell supernatants or biological fluids diluted in PBS without Ca and Mg + 2% BSA were added and then incubated for 2 hours at 37°C.
  5. The plates were washed five times with washing buffer, and then 100µl of PAb to PTX3 (human) (Biotin) (Prod. No. ALX-210-365B) 50ng/ml in washing buffer were added in each well.
  6. The plates were incubated for 1 hour at 37°C and then washed five times with 300µl of washing buffer.
  7. 100 µl/well streptavidin-horseradish peroxidase diluted 1:4’000 were added and the plates incubated for 1 hour at room temperature.
  8. After incubation the plates were washed 5 times with washing buffer and 100µl of TMB substrate solution was added to each well. After incubation for 10 min. at room-temperature, 50μl of stop-solution (1M H2SO4) was added. Subsequently, absorbance was read within 30min at 450nm.

Note: Do not use heparin plasma or serum samples with this assay. EDTA samples are recommended. The detection value in serum is generally less than in plasma samples.

 
PTX3 monoclonal antibody (MNB4) image
Figure: Dendritic cells that phagocyte Aspergillus fumigatus stained with MAb to PTX3 (MNB4) (Prod. No. ALX-804-464)."
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PTX3 monoclonal antibody (MNB4) image

Product Literature References

Pentraxin 3 Is Closely Associated With Tubulointerstitial Injury in Lupus Nephritis: A Large Multicenter Cross-Sectional Study: Y. Pang, et al.; Medicine (Baltimore) 95, e2520 (2016), Application(s): Used in ELISA measuring serum and urine concentrations of PTX3, Abstract; Full Text
Atorvastatin reduces long pentraxin 3 expression in vascular cells by inhibiting protein geranylgeranylation: R. Baetta, et al.; Vascul. Pharmacol. 67-69, 38 (2015), Application(s): Immunohistochemistry, Abstract;
Inflammation and thrombosis in essential thrombocythemia and polycythemia vera: different role of C-reactive protein and pentraxin 3: T. Barbui, et al.; Haematologica 96, 315 (2011), Abstract; Full Text
Endogenous PTX3 translocates at the membrane of late apoptotic human neutrophils and is involved in their engulfment by macrophages: S. Jaillon, et al.; Cell Death Differ. 16, 465 (2009), Abstract;
Pentraxin 3 is elevated in haemodialysis patients and is associated with cardiovascular disease: M. Boehme, et al.; Nephrol. Dial. Transplant. 22, 2224 (2007), Abstract;
The humoral pattern recognition receptor PTX3 is stored in neutrophil granules and localizes in extracellular traps: S. Jaillon, et al.; J. Exp. Med. 204, 793 (2007), Abstract;
The long Pentraxin Ptx3 is synthesized in IgA glomerulonephritis and Activates Mesangial Cells: B. Bussolati, et al.; J. Immunol. 170, 1466 (2003), Abstract;
Production of the Long Pentraxin PTX3 in Advanced Atherosclerotic Plaques: M.S. Rolph, et al.; Arterioscler. Thromb. Vasc. Biol. 22, e10 (2002), Abstract;
PTX3, A Prototypical Long Pentraxin, is an Early Indicator of Acute Myocardial Infarction in Humans: G. Peri, et al.; Circulation 102, 636 (2000), Abstract; Full Text
Multimer formation and ligand recognition by the long pentraxin PTX3. Similarities and differences with the short pentraxins C-reactive protein and serum amyloid P component: B. Bottazzi, et al.; J. Biol. Chem. 272, 32817 (1997), Abstract;

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