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Proteinase inhibitor 9 (human) monoclonal antibody (PI9-17)

 
ALX-804-457-C125 125 µg 473.00 USD
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Product Specification

Alternative Name:PI9, Serpin B9, CAP3, Cytoplasmic antiproteinase 3
 
Clone:PI9-17
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant human full-length PI9 (proteinase inhibitor 9).
 
UniProt ID:P50453
 
Species reactivity:Human
 
Crossreactivity:Does not cross-react with PI6, PI8 or PAI-2.
 
Applications:IHC (PS), WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (formaldehyde-fixed paraffin-embedded human tissues after pretreatment with 10mM citric acid pH 6.0 for 20min; use ~1:100. For fixed cytospin preparations fix cells for 10min in 10% formalin and then treat them as paraffin sections)
Western Blot (standard procedures)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects bands of ~42kDa (native PI9) and ~67kDa (PI9 complexed to granzyme B) by Western blot.
Not suitable for Flow Cytometry.
 
Positive Control:YT-Indy cells (a natural killer leukemia cell line) can be used as positive control. In other cell lines a non-specific protein band of ~63kDa can be detected.
 
Formulation:Liquid. In serum free culture supernatant containing 0.7% BSA and 0.1% sodium azide.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:The human intracellular serpin proteinase inhibitor 9 (PI9) is the only human protein able to inhibit the activity of the serine protease granzyme B. Granzyme B is expressed by cytotoxic lymphocytes and induces rapid target cell apoptosis. 
 
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Figure 1: Tissue sections of T-ALCL a) stained with MAb to PI9 (Prod. No. ALX-804-457) and b) double-stained with MAb to PI9 (red) (Prod. No. ALX-804-457) and granzyme B (brown). In panel b the arrows point to tumor-infiltrating CTLs positive for granzyme B and the arrowhead denotes an example of a PI9+ neoplastic cell.
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Figure 2: The following tumors were stained for PI9: a) B-CLL and b) classical Hodgkin disease, nodular sclerosing subtype.
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Product Literature References

Ectopic expression of the serine protease inhibitor PI9 modulates death receptor-mediated apoptosis: J.A. Kummer, et al.; Cell Death Differ. 14, 1486 (2007), Abstract;
Expression of the apoptosis inhibitor protease inhibitor 9 predicts clinical outcome in vaccinated patients with stage III and IV melanoma: I.S. van Houdt, et al.; Clin. Cancer Res. 11, 6400 (2005), Abstract; Full Text
High numbers of tumour-infiltrating activated cytotoxic T lymphocytes, and frequent loss of HLA class I and II expression, are features of aggressive B cell lymphomas of the brain and testis: S.A. Riemersma, et al.; J. Pathol. 206, 328 (2005), Abstract; Full Text
Hyperexpression of the granzyme B inhibitor PI-9 in human renal allografts: a potential mechanism for stable renal function in patients with subclinical rejection: A.T. Rowshani, et al.; Kidney Int. 66, 1417 (2004), Abstract; Full Text
Expression of the granzyme B inhibitor, protease inhibitor 9, by tumor cells in patients with non-Hodgkin and Hodgkin lymphoma: a novel protective mechanism for tumor cells to circumvent the immune system?: B.A. Bladergroen, et al.; Blood 99, 232 (2002), Abstract; Full Text
Modulators of inflammation use nuclear factor-kappa B and activator protein-1 sites to induce the caspase-1 and granzyme B inhibitor, proteinase inhibitor 9: P. Kannan-Thulasiraman & D.J. Shapiro; J. Biol. Chem. 277, 41230 (2002), Abstract; Full Text
The granzyme B inhibitor, protease inhibitor 9, is mainly expressed by dendritic cells and at immune-privileged sites: B.A. Bladergroen, et al.; J. Immunol. 166, 3218 (2001), Abstract; Full Text

General Literature References

Caspase-1 (interleukin-1beta-converting enzyme) is inhibited by the human serpin analogue proteinase inhibitor 9: R.R. Annand, et al.; Biochem. J. 342 Pt 3, 655 (1999), Abstract; Full Text
Inhibition of neutrophil elastase by recombinant human proteinase inhibitor 9: J.R. Dahlen, et al.; Biochim. Biophys. Acta 1451, 233 (1999), Abstract;
Selective regulation of apoptosis: the cytotoxic lymphocyte serpin proteinase inhibitor 9 protects against granzyme B-mediated apoptosis without perturbing the Fas cell death pathway: C.H. Bird, et al.; Mol. Cell. Biol. 18, 6387 (1998), Abstract;
Human proteinase inhibitor 9 (PI9) is a potent inhibitor of subtilisin A: J.R. Dahlen, et al.; BBRC 238, 329 (1997), Abstract;

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