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Caspase-8 (mouse) monoclonal antibody (3B10)

 
ALX-804-448-C100 100 µg 313.00 USD
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Product Specification

Alternative Name:FLICE
 
Clone:3B10
 
Host:Rat
 
Isotype:IgG1
 
Immunogen:Recombinant mouse caspase-8 p18 subunit.
 
UniProt ID:O89110
 
Species reactivity:Mouse
 
Crossreactivity:Does not cross-react with human caspase-8.
 
Applications:ELISA, Flow Cytometry, ICC, WB
 
Application Notes:ELISA: recombinant mouse caspase-8
Flow Cytometry: overexpressed casepase-8
Excellent for Immunocytochemistry.
Detects bands of ~55kDa (full-length caspase-8) and ~18kDa (apoptosis-induced cleavage fragment) by Western blot.
 
Purity Detail:Protein G-affinity purified.
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Handling:For long term storage, aliquot and freeze at -20°C. Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
804-447 448-1
Figure 1: Flow cytometry of HEK 293T cells transfected with FLAG tagged mutated mouse caspase-8.Method: MAbs 1G12 and 3B10 both detect overexpressed FLAG-epitope-tagged pro-caspase-8 (active-site-mutant) by flow cytometry in permeabilized cells. As a positive control staining with a MAb to FLAG was performed. As a negative control an isotype control was employed.
804-447 448-2
Figure 2: MAbs 1G12 and 3B10 both detect pro-caspase-8 in MEFs from WT mice, but not in MEFs from caspase-8-/- mice. Several smaller bands detected in the caspase-8-/- MEFs, correspond to truncated forms of caspase-8 made in the caspase-8-/- mice since only exons 1 and 2 of mouse caspase-8 were deleted in these knock-out mice and not the region encoding the p18 subunit.Note: Extra bands marked by * are only seen in lysates from caspase-8-/- MEFs and not in lysates from any WT cell lines or mouse WT tissue. Both MAbs to caspase-8 (mouse) do not recognize human caspase-8, whereas endogenous caspase-8 can be efficiently detected in various mouse cell lines. Upon an apoptotic stimulus e.g. by cross-linked rhsFasL both MAbs to caspase-8 (mouse) do also recognize the cleaved active p18 subunit of mouse caspase-8 in addition to the caspase-8 precursor.
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Product Literature References

Caspase-8 deficiency in epidermal keratinocytes triggers an inflammatory skin disease: A. Kovalenko, et al.; J. Exp. Med. 206, 2161 (2009), Abstract; Full Text
Impaired lactation in mice expressing dominant-negative FADD in mammary epithelium.: M. Shackleton, et al.; Dev. Dyn. 238, 1010 (2009), Abstract;
Membrane-bound Fas Ligand Only is Essential for Fas-Induced Apoptosis.: L.A. O’ Reilly, et al.; Nature 461, 659 (2009), Abstract;
Stimulation of Toll-like receptor 3 and 4 induces interleukin-1β maturation by caspase-8: J. Maelfait, et al.; J. Exp. Med. 205, 1967 (2008), Abstract; Full Text
Modifications and intracellular trafficking of FADD/MORT1 and caspase-8 after stimulation of T lymphocytes: L.A. O'Reilly; Cell Death Differ. 11, 724 (2004), Abstract;

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