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Caspase-8 (mouse) monoclonal antibody (1G12)

 
ALX-804-447-C100 100 µg 281.00 USD
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Product Specification

Alternative Name:FLICE
 
Clone:1G12
 
Host:Rat
 
Isotype:IgG1
 
Immunogen:Recombinant mouse caspase-8 p18 subunit .
 
UniProt ID:O89110
 
Species reactivity:Mouse
 
Specificity:Recognizes the p18 subunit of caspase-8.
 
Crossreactivity:Does not cross-react with human caspase-8.
 
Applications:ELISA, Flow Cytometry, ICC, WB
 
Application Notes:ELISA: recombinant mouse caspase-8
Flow Cytometry: overexpressed casepase-8
Excellent for Western blot.
Detects bands of ~55kDa (full-length caspase-8) and ~18kDa (apoptosis-induced cleavage fragment) by Western blot.
 
Purity Detail:Protein G-affinity purified.
 
Formulation:Liquid. In PBS containing 0.02% sodium azide and 50% glycerol.
 
Handling:For long term storage, aliquot and freeze at -20°C. Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
804-447 448-1
Figure 1: Flow cytometry of HEK 293T cells transfected with FLAG tagged mutated mouse caspase-8.Method: MAbs 1G12 and 3B10 both detect overexpressed FLAG-epitope-tagged pro-caspase-8 (active-site-mutant) by flow cytometry in permeabilized cells. As a positive control staining with a MAb to FLAG was performed. As a negative control an isotype control was employed.
804-447 448-2
Figure 2: MAbs 1G12 and 3B10 both detect pro-caspase-8 in MEFs from WT mice, but not in MEFs from caspase-8-/- mice. Several smaller bands detected in the caspase-8-/- MEFs, correspond to truncated forms of caspase-8 made in the caspase-8-/- mice since only exons 1 and 2 of mouse caspase-8 were deleted in these knock-out mice and not the region encoding the p18 subunit.Note: Extra bands marked by * are only seen in lysates from caspase-8-/- MEFs and not in lysates from any WT cell lines or mouse WT tissue. Both MAbs to caspase-8 (mouse) do not recognize human caspase-8, whereas endogenous caspase-8 can be efficiently detected in various mouse cell lines. Upon an apoptotic stimulus e.g. by cross-linked rhsFasL both MAbs to caspase-8 (mouse) do also recognize the cleaved active p18 subunit of mouse caspase-8 in addition to the caspase-8 precursor.
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Product Literature References

Short form FLICE-inhibitory protein promotes TNFα-induced necroptosis in fibroblasts derived from CFLARs transgenic mice: R. Shindo, et al.; Biochem. Biophys. Res. Commun. 480, 23 (2016), Application(s): Effect on signaling molecules leading to necroptosis, Abstract;
NIK promotes tissue destruction independently of the alternative NF-κB pathway through TNFR1/RIP1-induced apoptosis: L. Boutaffala, et al.; Cell. Death Differ. 22, 2020 (2015), Application(s): Immunoprecipitation, Abstract;
Punicalagin exerts protective effect against high glucose-induced cellular stress and neural tube defects: J. Zhong, et al.; Biochem. Biophys. Res. Commun. 467, 179 (2015), Application(s): Western Blot, Abstract;
Cellular-FLIP, Raji isoform (c-FLIP R) modulates cell death induction upon T-cell activation and infection: T. Telieps, et al.; Eur. J. Immunol. 43, 1499 (2013), Application(s): WB using mouse tissue homogenate, Abstract; Full Text
AIM2/ASC triggers caspase-8-dependent apoptosis in Francisella-infected caspase-1-deficient macrophages: R. Pierini, et al.; Cell Death Differ. 19, 1709 (2012), Abstract;
Protease Activity of Procaspase-8 Is Essential for Cell Survival by Inhibiting Both Apoptotic and Nonapoptotic Cell Death Dependent on Receptor-interacting Protein Kinase 1 (RIP1) and RIP3: M. Kikuchi, et al.; J. Biol. Chem. 287, 41165 (2012), Abstract;
Mechanisms of necroptosis in T cells: I.L. Ch’en, et al.; J. Exp. Med. 208, 633 (2011), Abstract;
Apoptosis and non-inflammatory phagocytosis can be induced by mitochondrial damage without caspases: M.F. Van Delft, et al.; Cell Death Differ. 17, 821 (2010), Application(s): WB using mouse thymocytes cell lysate, Abstract; Full Text
The Death Domain of FADD Is Essential for Embryogenesis, Lymphocyte Development, and Proliferation: H.Z. Imtiyaz, et al.; J. Biol. Chem. 284, 9917 (2009), Abstract; Full Text
A cell-type-specific requirement for IFN regulatory factor 5 (IRF5) in Fas-induced apoptosis: A. Couzinet, et al.; PNAS 105, 2556 (2008), Abstract; Full Text
Antigen-mediated T cell expansion regulated by parallel pathways of death: I.L. Ch’en, et al.; PNAS 105, 17463 (2008), Abstract; Full Text
Mutation of a Self-Processing Site in Caspase-8 Compromises Its Apoptotic but Not Its Nonapoptotic Functions in Bacterial Artificial Chromosome-Transgenic Mice: T.-B. Kang, et al.; J. Immunol. 181, 2522 (2008), Abstract;
Blockade of Tumor Necrosis Factor-induced Bid Cleavage by Caspase-resistant Rb: X. Huang, et al.; J. Biol. Chem. 282, 29401 (2007), Abstract; Full Text
FLIP(L) protects neurons against in vivo ischemia and in vitro glucose deprivation-induced cell death: E. Taoufik, et al.; J. Neurosci. 27, 6633 (2007), Abstract; Full Text
Apaf-1 and caspase-9 are required for cytokine withdrawal-induced apoptosis of mast cells but dispensable for their functional and clonogenic death: V.S. Marsden, et al.; Blood 107, 1872 (2006), Abstract; Full Text
Caspase-8 promotes cell motility and calpain activity under nonapoptotic conditions: B. Helfer, et al.; Cancer Res. 66, 4273 (2006), Abstract; Full Text
Loss of Caspase-9 Provides Genetic Evidence for the Type I/II Concept of CD95-mediated Apoptosis: A.K. Samraj, et al.; J. Biol. Chem. 281, 29652 (2006), Abstract; Full Text
Role of membrane sphingomyelin and ceramide in platform formation for Fas-mediated apoptosis: M. Miyaji, et al.; J. Exp. Med. 202, 249 (2005), Abstract; Full Text
Structural Requirements for Signal-induced Target Binding of FADD Determined by Functional Reconstitution of FADD Deficiency: H.Z. Imtiyaz, et al.; J. Biol. Chem. 280, 31360 (2005), Abstract; Full Text
Apaf-1 and caspase-9 do not act as tumor suppressors in myc-induced lymphomagenesis or mouse embryo fibroblast transformation: C.L. Scott, et al.; J. Cell Biol. 164, 89 (2004), Abstract; Full Text
Caspase-8 serves both apoptotic and nonapoptotic roles: T.B. Kang, et al.; J. Immunol. 173, 2976 (2004), Abstract; Full Text
Modifications and intracellular trafficking of FADD/MORT1 and caspase-8 after stimulation of T lymphocytes: L.A. O'Reilly; Cell Death Differ. 11, 724 (2004), Abstract; Full Text

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