Product Details
| Alternative Name: | FLICE |
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| Clone: | 1G12 |
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| Host: | Rat |
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| Isotype: | IgG1 |
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| Immunogen: | Recombinant mouse p20 caspase-8 subunit. |
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| UniProt ID: | O89110 |
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| Species reactivity: | Mouse
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| Specificity: | Recognizes the p18 subunit of caspase-8. |
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| Crossreactivity: | Does not cross-react with human caspase-8. |
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| Applications: | ELISA, Flow Cytometry, ICC, WB
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| Application Notes: | ELISA: recombinant mouse caspase-8
Flow Cytometry: overexpressed casepase-8
Excellent for Western blot.
Detects bands of ~55kDa (full-length caspase-8) and ~18kDa (apoptosis-induced cleavage fragment) by Western blot. |
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| Purity Detail: | Protein G-affinity purified. |
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| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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| Handling: | For long term storage, aliquot and freeze at -20°C. Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Figure 1: Flow cytometry of HEK 293T cells transfected with FLAG tagged mutated mouse caspase-8.Method: MAbs 1G12 and 3B10 both detect overexpressed FLAG-epitope-tagged pro-caspase-8 (active-site-mutant) by flow cytometry in permeabilized cells. As a positive control staining with a MAb to FLAG was performed. As a negative control an isotype control was employed.
Figure 2: MAbs 1G12 and 3B10 both detect pro-caspase-8 in MEFs from WT mice, but not in MEFs from caspase-8-/- mice. Several smaller bands detected in the caspase-8-/- MEFs, correspond to truncated forms of caspase-8 made in the caspase-8-/- mice since only exons 1 and 2 of mouse caspase-8 were deleted in these knock-out mice and not the region encoding the p18 subunit.Note: Extra bands marked by * are only seen in lysates from caspase-8-/- MEFs and not in lysates from any WT cell lines or mouse WT tissue. Both MAbs to caspase-8 (mouse) do not recognize human caspase-8, whereas endogenous caspase-8 can be efficiently detected in various mouse cell lines. Upon an apoptotic stimulus e.g. by cross-linked rhsFasL both MAbs to caspase-8 (mouse) do also recognize the cleaved active p18 subunit of mouse caspase-8 in addition to the caspase-8 precursor.
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Product Literature References
Proteasome inhibition triggers the formation of TRAIL receptor 2 platforms for caspase-8 activation that accumulate in the cytosol: C.T. Hellwig, et al.; Cell Death Differ.
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The non-apoptotic function of Caspase-8 in negatively regulating the CDK9-mediated Ser2 phosphorylation of RNA polymerase II in cervical cancer: R. Mandal, et al.; Cell. Mol. Life Sci.
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3-O-acetylrubianol C (3AR-C) Induces RIPK1-dependent Programmed Cell Death by Selective Inhibition of IKKβ: K. Kang, et al.; FASEB J.
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Beclin 1 Functions as a Negative Modulator of MLKL Oligomerisation by Integrating Into the Necrosome Complex: J. Seo, et al.; Cell Death Differ.
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Caspase-8, receptor-interacting protein kinase 1 (RIPK1), and RIPK3 regulate retinoic acid-induced cell differentiation and necroptosis: M. Someda, et al.; Cell Death Differ.
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TNF Signaling Dictates Myeloid and Non-Myeloid Cell Crosstalk to Execute MCMV-Induced Extrinsic Apoptosis: P. Mandal, et al.; Viruses
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Ubiquitin Ligases cIAP1 and cIAP2 Limit Cell Death to Prevent Inflammation: J. Zhang, et al.; Cell Rep.
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Ubiquitination of RIPK1 suppresses programmed cell death by regulating RIPK1 kinase activation during embryogenesis: X. Zhang, et al.; Nat. Commun.
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ABIN-1 regulates RIPK1 activation by linking Met1 ubiquitylation with Lys63 deubiquitylation in TNF-RSC: SA. Dziedzic, et al.; Nat. Cell Biol.
20, 58 (2018),
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Caspase-8 Collaborates with Caspase-11 to Drive Tissue Damage and Execution of Endotoxic Shock: P. Mandal, et al.; Immunity
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Cutting Edge: Mitochondrial Assembly of the NLRP3 Inflammasome Complex Is Initiated at Priming: E.I. Elliott, et al.; J. Immunol.
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Embryonic Lethality and Host Immunity of RelA-Deficient Mice Are Mediated by Both Apoptosis and Necroptosis: C. Xu, et al.; J. Immunol.
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The regulation of combined treatment-induced cell death with recombinant TRAIL and bortezomib through TRAIL signaling in TRAIL-resistant cells: S. Ryu, et al.; BMC Cancer
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Caspase-1 Engagement and TLR-Induced c-FLIP Expression Suppress ASC/Caspase-8-Dependent Apoptosis by Inflammasome Sensors NLRP1b and NLRC4: N. Van Opdenbosch, et al.; Cell Rep.
21, 3427 (2017),
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IFN-γ extends the immune functions of Guanylate Binding Proteins to inflammasome-independent antibacterial activities during Francisella novicida infection: P. Wallet, et al.; PLoS Pathog.
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Full Text
The linear ubiquitin chain assembly complex regulates TRAIL-induced gene activation and cell death: E. Lafont, et al.; EMBO J.
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Full Text
CHIP controls necroptosis through ubiquitylation- and lysosome-dependent degradation of RIPK3: J. Seo, et al.; Nat. Cell Biol.
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K45A mutation of RIPK1 results in poor necroptosis and cytokine signaling in macrophages, which impacts inflammatory responses in vivo: B. Shutinoski, et al.; Cell Death Differ.
23, 1628 (2016),
Abstract;
Full Text
Short form FLICE-inhibitory protein promotes TNFα-induced necroptosis in fibroblasts derived from CFLARs transgenic mice: R. Shindo, et al.; Biochem. Biophys. Res. Commun.
480, 23 (2016),
Application(s): Effect on signaling molecules leading to necroptosis,
Abstract;
The necroptosis-inducing kinase RIPK3 dampens adipose tissue inflammation and glucose intolerance: J. Gautheron, et al.; Nat. Commun.
7, 11869 (2016),
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Full Text
Using Förster-Resonance Energy Transfer to Measure Protein Interactions Between Bcl-2 Family Proteins on Mitochondrial Membranes: J.P. Pogmore, et al.; Methods Mol. Biol.
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Atg13 Is Essential for Autophagy and Cardiac Development in Mice: T. Kaizuka, et al.; Mol. Cell. Biol.
36, 585 (2015),
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Full Text
NIK promotes tissue destruction independently of the alternative NF-κB pathway through TNFR1/RIP1-induced apoptosis: L. Boutaffala, et al.; Cell. Death Differ.
22, 2020 (2015),
Application(s): Immunoprecipitation,
Abstract;
Punicalagin exerts protective effect against high glucose-induced cellular stress and neural tube defects: J. Zhong, et al.; Biochem. Biophys. Res. Commun.
467, 179 (2015),
Application(s): Western Blot,
Abstract;
RIP1-dependent Bid cleavage mediates TNFα-induced but Caspase-3-independent cell death in L929 fibroblastoma cells: G. Chen, et al.; Apoptosis
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Abstract;
FADD and caspase-8 mediate priming and activation of the canonical and noncanonical Nlrp3 inflammasomes: P. Gurung, et al.; J. Immunol.
192, 1835 (2014),
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Full Text
Cellular-FLIP, Raji isoform (c-FLIP R) modulates cell death induction upon T-cell activation and infection: T. Telieps, et al.; Eur. J. Immunol.
43, 1499 (2013),
Application(s): WB using mouse tissue homogenate,
Abstract;
Full Text
AIM2/ASC triggers caspase-8-dependent apoptosis in Francisella-infected caspase-1-deficient macrophages: R. Pierini, et al.; Cell Death Differ.
19, 1709 (2012),
Abstract;
Protease Activity of Procaspase-8 Is Essential for Cell Survival by Inhibiting Both Apoptotic and Nonapoptotic Cell Death Dependent on Receptor-interacting Protein Kinase 1 (RIP1) and RIP3: M. Kikuchi, et al.; J. Biol. Chem.
287, 41165 (2012),
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Mechanisms of necroptosis in T cells: I.L. Ch’en, et al.; J. Exp. Med.
208, 633 (2011),
Abstract;
Apoptosis and non-inflammatory phagocytosis can be induced by mitochondrial damage without caspases: M.F. Van Delft, et al.; Cell Death Differ.
17, 821 (2010),
Application(s): WB using mouse thymocytes cell lysate,
Abstract;
Full Text
The Death Domain of FADD Is Essential for Embryogenesis, Lymphocyte Development, and Proliferation: H.Z. Imtiyaz, et al.; J. Biol. Chem.
284, 9917 (2009),
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Full Text
A cell-type-specific requirement for IFN regulatory factor 5 (IRF5) in Fas-induced apoptosis: A. Couzinet, et al.; PNAS
105, 2556 (2008),
Abstract;
Full Text
Antigen-mediated T cell expansion regulated by parallel pathways of death: I.L. Ch’en, et al.; PNAS
105, 17463 (2008),
Abstract;
Full Text
Mutation of a Self-Processing Site in Caspase-8 Compromises Its Apoptotic but Not Its Nonapoptotic Functions in Bacterial Artificial Chromosome-Transgenic Mice: T.-B. Kang, et al.; J. Immunol.
181, 2522 (2008),
Abstract;
Blockade of Tumor Necrosis Factor-induced Bid Cleavage by Caspase-resistant Rb: X. Huang, et al.; J. Biol. Chem.
282, 29401 (2007),
Abstract;
Full Text
FLIP(L) protects neurons against in vivo ischemia and in vitro glucose deprivation-induced cell death: E. Taoufik, et al.; J. Neurosci.
27, 6633 (2007),
Abstract;
Full Text
Apaf-1 and caspase-9 are required for cytokine withdrawal-induced apoptosis of mast cells but dispensable for their functional and clonogenic death: V.S. Marsden, et al.; Blood
107, 1872 (2006),
Abstract;
Full Text
Caspase-8 promotes cell motility and calpain activity under nonapoptotic conditions: B. Helfer, et al.; Cancer Res.
66, 4273 (2006),
Abstract;
Full Text
Loss of Caspase-9 Provides Genetic Evidence for the Type I/II Concept of CD95-mediated Apoptosis: A.K. Samraj, et al.; J. Biol. Chem.
281, 29652 (2006),
Abstract;
Full Text
Role of membrane sphingomyelin and ceramide in platform formation for Fas-mediated apoptosis: M. Miyaji, et al.; J. Exp. Med.
202, 249 (2005),
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Full Text
Structural Requirements for Signal-induced Target Binding of FADD Determined by Functional Reconstitution of FADD Deficiency: H.Z. Imtiyaz, et al.; J. Biol. Chem.
280, 31360 (2005),
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Full Text
Apaf-1 and caspase-9 do not act as tumor suppressors in myc-induced lymphomagenesis or mouse embryo fibroblast transformation: C.L. Scott, et al.; J. Cell Biol.
164, 89 (2004),
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Full Text
Caspase-8 serves both apoptotic and nonapoptotic roles: T.B. Kang, et al.; J. Immunol.
173, 2976 (2004),
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Full Text
Modifications and intracellular trafficking of FADD/MORT1 and caspase-8 after stimulation of T lymphocytes: L.A. O'Reilly; Cell Death Differ.
11, 724 (2004),
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Full Text
