Product Specification
| Alternative Name: | TLR4/MD-2 |
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| Clone: | MTS510 |
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| Host: | Rat |
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| Isotype: | IgG2a |
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| Immunogen: | Ba/F3 cells expressing mouse TLR4 and MD-2 complex. |
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| UniProt ID: | Q9QUK6, Q8K2T5 (TLR4), Q9JHF9 (MD-2) |
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| GenBank ID: | AAH29856 |
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| Species reactivity: | Mouse
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| Specificity: | Recognizes the TLR4/MD-2 complex. |
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| Applications: | Flow Cytometry, IP, FUNC
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| Application Notes: | Functional Application: inhibition of LPS-induced cytokine production; use preservative free formulation. |
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| Formulation: | Liquid. In PBS. |
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| Use/Stability: | Stable for at least 1 year after receipt when stored as recommended. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Shipped on Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Technical Info/Product Notes: | Replacement for ADI-CSA-805. |
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Figure: FACS analysis using MAb to to Toll-like Receptor 4/MD-2 (mouse) (MTS510) (Prod. No.
ALX-804-430).
Method: Mouse BaF3 cells (5x10
5) expressing surface mTLR4 alone or together with the accessory protein MD-2 or BaF3 parental cells, were incubated on ice for 30min. in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1µg/ml of MAb to Toll-like Receptor 4/MD-2 (mouse) (MTS510). After washing in FACS buffer, FITC-conjugated antibody to rat IgG (Prod. No.
ALX-211-051) was added. Cells were incubated on ice for 30 min., washed and then analyzed by flow cytometry.
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Product Literature References
The role of TLR2 in the inflammatory activation of mouse fibroblasts by human antiphospholipid antibodies: N. Satta, et al.; Blood
109, 1507 (2007),
Abstract;
Cutting edge: cell surface expression and lipopolysaccharide signaling via the toll-like receptor 4-MD-2 complex on mouse peritoneal macrophages: S. Akashi, et al.; J. Immunol.
164, 3471 (2000),
Abstract;
Full Text
Cutting edge: endotoxin tolerance in mouse peritoneal macrophages correlates with down-regulation of surface toll-like receptor 4 expression: F. Nomura, et al.; J. Immunol.
164, 3476 (2000),
Abstract;
Full Text
Synergy and cross-tolerance between toll-like receptor (TLR) 2- and TLR4-mediated signaling pathways: S. Sato, et al.; J. Immunol.
165, 7096 (2000),
Abstract;
Full Text
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