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Toll-like receptor 4/MD-2 (mouse) monoclonal antibody (MTS510)

ALX-804-430-C100 100 µg Purified 250.00 USD
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Product Specification

Alternative Name:TLR4/MD-2
Immunogen:Ba/F3 cells expressing mouse TLR4 and MD-2 complex.
UniProt ID:Q9QUK6, Q8K2T5 (TLR4), Q9JHF9 (MD-2)
GenBank ID:AAH29856
Species reactivity:Mouse
Specificity:Recognizes the TLR4/MD-2 complex.
Applications:Flow Cytometry, IP
Formulation:Liquid. In PBS containing 0.02% sodium azide.
Use/Stability:Stable for at least 1 year after receipt when stored as recommended.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Technical Info/Product Notes:Replacement for ADI-CSA-805.
Figure: FACS analysis using MAb to to Toll-like Receptor 4/MD-2 (mouse) (MTS510) (Prod. No. ALX-804-430).Method: Mouse BaF3 cells (5x105) expressing surface mTLR4 alone or together with the accessory protein MD-2 or BaF3 parental cells, were incubated on ice for 30min. in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1µg/ml of MAb to Toll-like Receptor 4/MD-2 (mouse) (MTS510). After washing in FACS buffer, FITC-conjugated antibody to rat IgG (Prod. No. ALX-211-051) was added. Cells were incubated on ice for 30 min., washed and then analyzed by flow cytometry.
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Product Literature References

The role of TLR2 in the inflammatory activation of mouse fibroblasts by human antiphospholipid antibodies: N. Satta, et al.; Blood 109, 1507 (2007), Abstract;
Cutting edge: cell surface expression and lipopolysaccharide signaling via the toll-like receptor 4-MD-2 complex on mouse peritoneal macrophages: S. Akashi, et al.; J. Immunol. 164, 3471 (2000), Abstract; Full Text
Cutting edge: endotoxin tolerance in mouse peritoneal macrophages correlates with down-regulation of surface toll-like receptor 4 expression: F. Nomura, et al.; J. Immunol. 164, 3476 (2000), Abstract; Full Text
Synergy and cross-tolerance between toll-like receptor (TLR) 2- and TLR4-mediated signaling pathways: S. Sato, et al.; J. Immunol. 165, 7096 (2000), Abstract; Full Text

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