Product Details
Alternative Name: | Class III β-tubulin |
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Clone: | TU-20 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Synthetic peptide corresponding to aa 441-448 (E441SESQGPK448) of human βIII-tubulin (class III β-tubulin). |
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UniProt ID: | Q13509 |
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Species reactivity: | Human
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Specificity: | Recognizes βIII-tubulin located in neurons. |
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Applications: | ELISA, Flow Cytometry, ICC, IHC (FS), IHC (PS), WB
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Recommended Dilutions/Conditions: | Immunohistochemistry (paraffin sections, 10µg/ml; pretreatment with 0.1% pepsin (trypsin) in 0.1M HCl; incubation for 30 min. at room temperature or high temperature citrate buffer antigen retrieval) Western Blot (1-2µg/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Formulation: | Liquid. In PBS, pH 7.4, containing 15 mM sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice Not Frozen |
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Short Term Storage: | +4°C |
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Long Term Storage: | +4°C |
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Technical Info/Product Notes: | Application Note:
Image-Based Analysis of a Human Neurosphere Stem Cell Model for the Evaluation of Potential Neurotoxicants |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
A high throughput embryonic stem cell screen identifies Oct-2 as a bifunctional regulator of neuronal differentiation: E. Theodorou, et al.; Genes Dev.
23, 575 (2009),
Abstract;
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