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cGMP-activated protein kinase substrate monoclonal antibody (7H1)

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Product Specification

Alternative Name:PKG substrate, Protein kinase G substrate
 
Clone:7H1
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Synthetic phosphopeptide corresponding to PKG (protein kinase G) substrate consensus phosphorylation site of activated PKG substrate conjugated to KLH.
 
UniProt ID:P13976 (human PRKG1), P13237 (human PRKG2)
 
Species reactivity:Species independent
 
Specificity:Recognizes RKVpSK motif of activated PKG substrate in a broad range of species.
 
Applications:ELISA
 
Recommended Dilutions/Conditions:ELISA (use at 0.1-1µg/ml). Do not use milk/casein for blocking and dilution! Replace by BSA! Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
 
Application Notes:ELISA: non-radioactive quantification of phospho-peptides in kinase/phosphatase assays.
Not suitable for Western blot!
 
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
 
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
 
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
 
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 3 months.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 

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