[pSer⁴⁷³]Akt, mAb (11E6)

[Protein kinase B, PKB]



ALX-804-401-C100	100 µg	474.00 USD

Product Specification

Purity Detail:	Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.

Formulation:	Lyophilized from 1ml 2x PBS containing 0.09% sodium azide, PEG and sucrose.

Clone:	11E6

Isotype:	Mouse IgG1

Immunogen:	Synthetic peptide corresponding to C-terminal (PQFpSYSA) of human Akt (protein kinase B; PKB) phosphorylated at Ser⁴⁷³.

Reconstitution:	Reconstitute with 1ml distilled water (15 min., RT).

Specificity:	Recognizes human, mouse, rat and dog Ser^472/473/474-phosphorylated Akt (see technical note). Does not cross-react with dephosphorylated Akt or other unrelated serine-phosphorylated proteins.

Application:	Flow Cytometry: (1μg/μm) Immunohistochemistry: Optimal conditions must be determined individually. A starting dilution of 5μg/ml is recommended. Western Blot: (0.5µg/ml for HRPO/ECL detection). Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride. Optimal conditions must be determined individually for each application.

Shipping:	SHIPPED ON BLUE ICE

Long Term Storage:	-20°C

Use/Stability:	Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.

Handling:	After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.

Background / Technical Information:	Technical Note: There are three mammalian isoforms of Akt: Akt1 (PKBα), Akt2 (PKBβ), and Akt3 (PKBγ). Akt1 shares 81% and 83% aa identity with Akt2 and Akt3, respectively. Although the three isoforms show broad tissue distribution, Akt1 is most ubiquitously expressed. Akt2 is expressed at a lower level than Akt1 except in insulin-responsive tissues where it predominates. Akt2 is more abundant and more highly activated than Akt1 in adipocytes. Akt3 is expressed at the lowest level except in testes and brain. The C-terminal sequence of the three isoforms are shown below. The phosphorylation site slightly differs, thus the phosphorylation site Ser⁴⁷³ of Akt1 corresponds to Ser⁴⁷⁴ in Akt2 and Ser⁴⁷² in Akt3. Akt1: V⁴⁶¹DSERRPHFPQFS⁴⁷³YSASSTA⁴⁸⁰ Swiss-Prot-Link: P31749Akt2: L⁴⁶¹LELDQRTHFPQFS⁴⁷⁴YSASIRE⁴⁸¹ Swiss-Prot-Link: P31751Akt3: D⁴⁶¹NERRPHFPQFS⁴⁷²YSASGRE⁴⁷⁹ Swiss-Prot-Link: Q9Y243 Replacement for ADI-905-658