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Hrs and Hrs-2 monoclonal antibody (A-5)

ALX-804-382-C050 50 µg 296.00 USD
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Product Specification

Alternative Name:Hepatocyte growth factor-regulated tyrosine kinase substrate, HGS
Immunogen:Recombinant full length rat Hrs-2 (hepatocyte growth factor-regulated tyrosine kinase substrate).
UniProt ID:Q9JJ50
Species reactivity:Human, Mouse, Rat
Specificity:Recognizes Hrs (HGS) and Hrs-2.
Applications:IHC, IP, WB
Recommended Dilutions/Conditions:Immunohistochemistry (1:100-1:1,000)
Immunoprecipitation (2µg/ml)
Western Blot (1:2,000-1:3,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Affinity-purified.
Formulation:Liquid. In 0.1M PBS, pH 7.2, containing 50% glycerol.
Use/Stability:Stable for 3 months when stored at +4°C.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Scientific Background:Hrs/Hgs (hepatocyte growth factor-regulated tyrosine kinase substrate) is a tyrosine-phosphorylated member of a protein family characterized by a conserved 3'-phosphoinositide-interacting RING zinc finger domain, the so called FYVE finger. A variant of Hrs, Hrs-2 is an ATPase that has been shown to play a regulatory role in the docking and/or fusion of synaptic vesicles to plasma membranes through a calcium-regulated interaction with SNAP-25. Hrs-2 physically interacts with Eps15, a protein required for receptor-mediated endocytosis. The Hrs-2/Eps15 interaction is calcium dependent, inhibited by SNAP-25 and alpha-adaptin, and results in the inhibition of receptor-mediated endocytosis. Immunoelectron microscopy reveals Hrs-2 localization on the limiting membrane of multivesicular bodies, organelles in the endosomal pathway. It is suggested that Hrs-2 acts to provide communication between endo- and exocytic processes.

Product Literature References

The Dynamics of TGF-β Signaling Are Dictated by Receptor Trafficking via the ESCRT Machinery: D.S.J. Miller, et al.; Cell Rep. 25, 1841 (2018), Abstract;
Hepatocyte growth factor-regulated tyrosine kinase substrate (HRS) interacts with PELP1 and activates MAPK: S.K. Rayala, et al.; J. Biol. Chem. 281, 4395 (2006), Abstract; Full Text
CART: an Hrs/actinin-4/BERP/myosin V protein complex required for efficient receptor recycling: Q. Yan, et al.; Mol. Biol. Cell 16, 2470 (2005), Abstract; Full Text
SNAP-25-associated Hrs-2 protein colocalizes with AQP2 in rat kidney collecting duct principal cells: A. Shukla, et al.; Am. J. Physiol. Renal Physiol. 281, F546 (2001), Abstract; Full Text
Distinct protein domains are responsible for the interaction of Hrs-2 with SNAP-25. The role of Hrs-2 in 7 S complex formation: S. Tsujimoto & A.J. Bean; J. Biol. Chem. 275, 2938 (2000), Abstract; Full Text
Hrs-2 regulates receptor-mediated endocytosis via interactions with Eps15: A.J. Bean, et al.; J. Biol. Chem. 275, 15271 (2000), Abstract; Full Text
Isoform-specific exocytotic protein mRNA expression in hypothalamic magnocellular neurons: regulation after osmotic challenge: G. Jacobsson, et al.; Neuroendocrinology 70, 392 (1999), Abstract;
The cellular and developmental expression of hrs-2 in rat: S. Tsujimoto, et al.; Eur. J. Neurosci. 11, 3047 (1999), Abstract;

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