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ATF6 monoclonal antibody (70B1413.1)

 
ALX-804-381-C100 100 µg 319.00 USD
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Product Specification

Alternative Name:Activating transcription factor 6
 
Clone:70B1413.1
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Partial human ATF6 (activating transcription factor 6) containing aa 1-273.
 
UniProt ID:P18850
 
Species reactivity:Human, Mouse, Rat
Hamster, Rabbit
 
Applications:ICC, IHC (PS), IP, WB
ChIP
 
Recommended Dilutions/Conditions:Western Blot (1-5µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects bands of ~90kDa (full length ATF6) and ~50-70kDa (cleaved forms of ATF6) by Western blot.
 
Purity Detail:Protein G-affinity purified.
 
Formulation:Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.
 
Use/Stability:Stable for 6 months when stored at +4°C.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
804-381
Figure 1: Western blot analysis of ATF6 on 15µg of (A) Jurkat and (B) NIH3T3 cell lysates using MAb to ATF6 (70B1413.1) (Prod. No. ALX-804-381). A band of ~90kDa was detected.
804-381 2
Figure 2: Western blot analysis of ATF6 using MAb to ATF6 (70B1413.1) (Prod. No. ALX-804-381). Method: Western blots were probed with 4µg/ml of MAb to ATF6 (70B1413.1), followed by an HRP-conjugated second step and visualized with PicoTect Western Blot Chemiluminescence Substrate. Film was exposed for 1 min. The top arrow corresponds to the ~90kDa form of ATF6 described as full-length in the literature. Lane 1: 293 cells transfected with full-length ATF6. Lane 2: 293 cells transfected with partial length ATF6 (aa 1-373). Lane 3: Untransfected 293 cells. The human full-length and partial length ATF6 plasmids are described in Luo and Lee (2002) [1].
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Product Literature References

Resveratrol triggers ER stress-mediated apoptosis by disrupting N-linked glycosylation of proteins in ovarian cancer cells: H. Gwak, et al. ; Cancer Lett. 371, 347 (2016), Application(s): Activation of ER stress sensors triggers cellular adaptation, Abstract;
Response of myeloma to the proteasome inhibitor bortezomib is correlated with the unfolded protein response regulator XBP-1: S.C. Ling, et al.; Haematologica 97, 64 (2012), Abstract;
ATF6alpha induces XBP1-independent expansion of the endoplasmic reticulum: H. Bommiasamy, et al.; J. Cell Sci. 122, 1626 (2009), Abstract;
Eif-2a protects brainstem motoneurons in a murine model of sleep apnea: Y. Zhu, et al.; J. Neurosci. 28, 2168 (2008), Abstract;
Acetaminophen induces ER dependent signaling in mouse liver: G. Nagy, et al.; Arch. Biochem. Biophys. 459, 273 (2007), Abstract;
Cardiomyocyte apoptosis in autoimmune cardiomyopathy: mediated via endoplasmic reticulum stress and exaggerated by norepinephrine: W. Mao, et al.; Am. J. Heart Circ. Physiol. 293, H1636 (2007), Abstract;
Regulation of ERGIC-53 gene transcription in response to endoplasmic reticulum stress: M. Renna, et al.; J. Biol. Chem. 282, 22499 (2007), Abstract; Full Text
3,3’-diindolylmethane (DIM) and its derivatives induce apoptosis in pancreatic cancer cells through endoplasmic reticulum stress-dependent upregulation of DR5: M. Abdelrahim, et al.; Carcinogenesis 27, 717 (2006), Abstract; Full Text
Cytoprotective gene bi-1 is required for intrinsic protection from endoplasmic reticulum stress and ischemia-reperfusion injury: B. Bailly-Maitre, et al.; PNAS 103, 2809 (2006), Abstract; Full Text
Dynamic recruitment of transcription factors and epigenetic changes on the ER stress response gene promoters: G. Donati, et al.; Nucleic Acids Res. 34, 3116 (2006), Abstract; Full Text
Endoplasmic reticulum stress and neurodegeneration in rats neonatally infected with borna disease virus: B.L. Williams and W.I. Lipkin; J. Virol. 80, 8613 (2006), Abstract;
Flavivirus infection activates the XBP1 pathway of the unfolded protein response to cope with endoplasmic reticulum stress: C.Y. Yu, et al.; J. Virol. 80, 11868 (2006), Abstract; Full Text
Glia-specific activation of all pathways of the unfolded protein response in vanishing white matter disease: B. van Kollenburg, et al.; J. Neuropathol. Exp. Neurol. 65, 707 (2006), Abstract;
Intramembrane proteolytic cleavage by human signal peptide peptidase like 3 and malaria signal peptide peptidase: A.C. Nyborg, et al.; FASEB J. 20, 1671 (2006), Abstract;
Intrinsic capacities of molecular sensors of the unfolded protein response to sense alternate forms of endoplasmic reticulum stress: J.B. DuRose, et al.; Mol. Biol. Cell 17, 3095 (2006), Abstract; Full Text
Involvement of a novel Q-SNARE, D12, in quality control of the endomembrane system: A.J. Okumura, et al.; J. Biol. Chem. 281, 4495 (2006), Abstract; Full Text
Nitric oxide-induced endoplasmic reticulum stress activates the expression of cargo receptor proteins and alters the glycoprotein transport to the Golgi complex: M. Renna, et al.; Int. J. Biochem. Cell Biol. 38, 2040 (2006), Abstract;
Spinal cord endoplasmic reticulum stress associated with a microsomal accumulation of mutant superoxide dismutase-1 in an ALS model: H. Kikuchi, et al.; PNAS 103, 6025 (2006), Abstract; Full Text
The unfolded protein response modulates toxicity of the expanded glutamine androgen receptor: M. Thomas, et al.; J. Biol. Chem. 280, 21264 (2005), Abstract; Full Text
A signal peptide peptidase (SPP) reporter activity assay based on the cleavage of type II membrane protein substrates provides further evidence for an inverted orientation of the SPP active site relative to presenilin: A.C. Nyborg, et al.; J. Biol. Chem. 279, 43148 (2004), Abstract; Full Text
Underglycosylation of ATF6 as a novel sensing mechanism for activation of the unfolded protein response: M. Hong, et al.; J. Biol. Chem. 279, 11354 (2004), Abstract; Full Text
Kaposi’s sarcoma-associated herpesvirus-infected primary effusion lymphoma has a plasma cell gene expression profile: R.G. Jenner, et al.; PNAS 100, 10399 (2003), Abstract;
Requirement of the p38 mitogen-activated protein kinase signalling pathway for the induction of the 78 kDa glucose-regulated protein/immunoglobulin heavy-chain binding protein by azetidine stress: activating transcription factor 6 as a target for stress-i: S. Luo and A.S. Lee; Biochem. J. 366, 787 (2002), Abstract; Full Text

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