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Dnmt3a (mouse) monoclonal antibody (64B1446)

 
ALX-804-370-C100 100 µg 334.00 USD
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Product Specification

Alternative Name:DNA methyltransferase-3a
 
Clone:64B1446
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant mouse Dnmt3a (DNA methyltransferase-3a).
 
UniProt ID:O88508
 
Species reactivity:Mouse
 
Specificity:Recognizes C-terminal epitope aa 705-908 of mouse Dnmt3a.
 
Crossreactivity:Weakly cross-reacts with human Dnmt3a.
 
Applications:ICC, IHC, IP, WB
ChIP
 
Recommended Dilutions/Conditions:Immunocytochemistry (5µg/ml)
Immunohistochemistry (1:400)
Western Blot (2µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein G-affinity purified.
 
Formulation:Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
804-370 1
Figure: Western blot analysis of Dnmt3a using ALX-804-370 at 2µg/ml against 10µg of 293 cell lysate transfected with either mouse Dnmt3a (lane A) or mouse Dnmt3b (lane B).
804-370 2
Figure: Immunostaining of Dnmt3a using ALX-804-370 at 5µg/ml dilution on cultured HeLa cells. The nucleic staining is shown in most of the cells.
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804-370 1 804-370 2

Product Literature References

Cell and stage of transformation-specific effects of folate deficiency on methionine cycle intermediates and DNA methylation in an in vitro model: J.M. Stempak, et al.; Carcinogenesis 26, 981 (2005), Abstract;
De novo DNA methyltransferases Dnmt3a and Dnmt3b primarily mediate the cytotoxic effect of 5-aza-2’-deoxycytidine: M. Oka, et al.; Oncogene 24, 3091 (2005), Abstract;
Dynamic expression of de novo DNA methyltransferases Dnmt3a and Dnmt3b in the central nervous system: J. Feng, et al.; J. Neurosci. Res. 79, 734 (2005), Abstract;
Myc represses transcription through recruitment of DNA methyltransferase corepressor: C. Brenner, et al.; EMBO 24, 336 (2005), Abstract;
Loss of estrogen receptor signaling triggers epigenetic silencing of downstream targets in breast cancer: Y.W. Leu, et al.; Cancer Res. 64, 8184 (2004), Abstract;
Association of Lsh, a regulator of DNA methylation, with pericentromeric heterochromatin is dependent on intact heterochromatin: Q. Yan, et al.; Mol. Cell. Biol. 23, 8416 (2003), Abstract; Full Text
A novel Dnmt3a isoform produced from an alternative promoter localizes to euchromatin and its expression correlates with active de novo methylation: T. Chen, et al.; J. Biol. Chem. 277, 38746 (2002), Abstract;
Lsh, a member of the SNF2 family, is required for genome-wide methylation: K. Dennis, et al.; Genes Dev. 15, 2940 (2001), Abstract; Full Text

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