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Toll-like receptor 9 monoclonal antibody (26C593.2) (R-PE conjugate)

ALX-804-364R-C100 100 µg R-PE 472.00 USD
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Product Specification

Alternative Name:TLR9, CD289
Immunogen:Synthetic peptide corresponding to aa 268-284 in the cytoplasmic portion of human TLR9 (Toll-like Receptor 9).
UniProt ID:Q9NR96
Species reactivity:Human, Mouse, Rat
Dog, Horse, Monkey
Applications:Flow Cytometry, IHC (FS), IHC (PS)
Recommended Dilutions/Conditions:Flow Cytometry (intracellular, 0.1-2µg/106 cells),Immunohistochemistry (frozen sections (10-20µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~120kDa by Western blot, may also detect a smaller band of TLR9 isoform B.
Flow Cytometry: Suitable for cell surface and intracellular FC.
Positive Control:Whole cell lysate from human peripheral blood monocyte cells (PBMC) or Daudi can be used as positive control.
Purity Detail:Protein G-affinity purified.
Formulation:Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.
Handling:Do not freeze. Protect from light.
Shipping:Shipped on Blue Ice
Long Term Storage:+4°C

Product Literature References

Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils: C.K. Wong, et al.; Am. J. Respir. Cell Mol. Biol. 37, 85 (2007), Abstract;
Surface expression of Toll-like receptor 9 is upregulated on intestinal epithelial cells in response to pathogenic bacterial DNA: J.B. Ewaschuk, et al.; Infect. Immun. 75, 2572 (2007), Abstract;
Activation of anti-hepatitis C virus responses via Toll-like receptor 7: J. Lee, et al.; PNAS 103, 1828 (2006), Abstract; Full Text
The Unc93b1 mutation 3d disrupts exogenous antigen presentation and signaling via Toll-like receptors 3, 7 and 9: K. Tabeta, et al.; Nat. Immunol. 7, 156 (2006), Abstract; Full Text
Toll-like receptor 9 (TLR9) is present in murine liver sinusoidal endothelial cells (LSECs) and mediates the effect of CpG-oligonucleotides: M. Martin-Armas, et al.; J. Hepatol. 44, 939 (2006), Abstract;
Toll-like receptors in cellular subsets of human tonsil T cells: altered expression during recurrent tonsillitis: A. Mansson, et al.; Respir Res. 7, 36 (2006), Abstract; Full Text
Evidence of Toll-like receptor molecules on human platelets: F. Cognasse, et al.; Immunol. Cell Biol. 83, 196 (2005), Abstract;
Expression of mRNA and proteins for toll-like receptors, associated molecules, defensins and LL-37 by SRIK-NKL, a CD8+ NK/T cell line: M.D. Srivastava and B.I. Srivastava; Leuk. Res. 29, 813 (2005), Abstract;
Human lung cancer cells express functionally active Toll-like receptor 9: D. Droemann, et al.; Respir. Res. 6, 1 (2005), Abstract; Full Text
TGF-alpha regulates TLR expression and function on epidermal keratinocytes: L.S. Miller, et al.; J. Immunol. 174, 6137 (2005), Abstract;
TLR-induced inflammation in cystic fibrosis and non-cystic fibrosis airway epithelial cells: C.M. Greene, et al.; J. Immunol. 174, 1638 (2005), Abstract; Full Text
CpG oligodeoxynucleotides activate HIV replication in latently infected human T cells: C. Scheller, et al.; J. Biol. Chem. 279, 21897 (2004), Abstract; Full Text
Cytoplasmic domain-mediated dimerizations of toll-like receptor 4 observed by beta-lactamase enzyme fragment complementation: H.K. Lee, et al.; J. Biol. Chem. 279, 10564 (2004), Abstract; Full Text

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