Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Toll-like receptor 9 monoclonal antibody (26C593.2)

ALX-804-364-C100 100 µg 411.00 USD
Do you need bulk/larger quantities?

Product Specification

Alternative Name:TLR9, CD289
Immunogen:Synthetic peptide corresponding to aa 268-284 in the cytoplasmic portion of human TLR9 (Toll-like Receptor 9).
UniProt ID:Q9NR96
Species reactivity:Human, Mouse, Rat
Dog, Horse, Monkey
Applications:Flow Cytometry, IHC (FS), IHC (PS), WB
Recommended Dilutions/Conditions:Flow Cytometry (intracellular, 0.1-2µg/106 cells)
Immunohistochemistry (frozen sections, 10-20µg/ml)
Western Blot (1-5µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Flow Cytometry: Suitable for cell surface and intracellular FC.
Detects a band of ~120kDa by Western blot, may also detect a smaller band of TLR9 isoform B.
Positive Control:Whole cell lysate from human peripheral blood monocyte cells (PBMC) or Daudi can be used as positive control.
Purity Detail:Protein G-affinity purified.
Formulation:Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
804-364 1 new
Figure 1: Western blot analysis of TLR9 using MAb to Toll-like Receptor 9 (human) (26C593.2) (Prod. No. ALX-804-364) at a dilution of 3µg/ml. A) human PBMC; B) human intestine; C) mouse intestine; D) rat intestine lysate; E) human MCF7 cell lysate (MAb dilution 5μg/ml).
804-364 2 new
Figure 2: Intracellular flow cytometric analysis of TLR9 in Ramos cells using 0.1μg MAb to Toll-like Receptor 9 (human) (26C593.2) (Prod. No. ALX-804-364). Shaded histogram represents Ramos cells without antibody; green represents isotype control (mouse IgG1); red represents MAb to Toll-like Receptor 9 (human) (26C593.2).
804-364 3 new
Figure 3: Immunohistochemical analysis of TLR9 in A) adenocarcinoma of the lung, C) A549 cells, and F) malignant lung tissues using MAb to Toll-like Receptor 9 (human) (26C593.2) (Prod. No. ALX-804-364) at 1:100 dilution, (600x magnification).
ALX-804-364 IHC
Immunohistochemistry analysis: Formalin-fixed, paraffin-embedded human spleen probed with Toll-like receptor 9, mAb (26C593.2) at 5µg/ml.
Please mouse over
804-364 1 new 804-364 2 new 804-364 3 new ALX-804-364 IHC

Product Literature References

Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils: C.K. Wong, et al.; Am. J. Respir. Cell Mol. Biol. 37, 85 (2007), Abstract;
Surface expression of Toll-like receptor 9 is upregulated on intestinal epithelial cells in response to pathogenic bacterial DNA: J.B. Ewaschuk, et al.; Infect. Immun. 75, 2572 (2007), Abstract;
Activation of anti-hepatitis C virus responses via Toll-like receptor 7: J. Lee, et al.; PNAS 103, 1828 (2006), Abstract; Full Text
The Unc93b1 mutation 3d disrupts exogenous antigen presentation and signaling via Toll-like receptors 3, 7 and 9: K. Tabeta, et al.; Nat. Immunol. 7, 156 (2006), Abstract; Full Text
Toll-like receptor 9 (TLR9) is present in murine liver sinusoidal endothelial cells (LSECs) and mediates the effect of CpG-oligonucleotides: M. Martin-Armas, et al.; J. Hepatol. 44, 939 (2006), Abstract;
Toll-like receptors in cellular subsets of human tonsil T cells: altered expression during recurrent tonsillitis: A. Mansson, et al.; Respir Res. 7, 36 (2006), Abstract; Full Text
Evidence of Toll-like receptor molecules on human platelets: F. Cognasse, et al.; Immunol. Cell Biol. 83, 196 (2005), Abstract;
Expression of mRNA and proteins for toll-like receptors, associated molecules, defensins and LL-37 by SRIK-NKL, a CD8+ NK/T cell line: M.D. Srivastava and B.I. Srivastava; Leuk. Res. 29, 813 (2005), Abstract;
Human lung cancer cells express functionally active Toll-like receptor 9: D. Droemann, et al.; Respir. Res. 6, 1 (2005), Abstract; Full Text
TGF-alpha regulates TLR expression and function on epidermal keratinocytes: L.S. Miller, et al.; J. Immunol. 174, 6137 (2005), Abstract;
TLR-induced inflammation in cystic fibrosis and non-cystic fibrosis airway epithelial cells: C.M. Greene, et al.; J. Immunol. 174, 1638 (2005), Abstract; Full Text
CpG oligodeoxynucleotides activate HIV replication in latently infected human T cells: C. Scheller, et al.; J. Biol. Chem. 279, 21897 (2004), Abstract; Full Text
Cytoplasmic domain-mediated dimerizations of toll-like receptor 4 observed by beta-lactamase enzyme fragment complementation: H.K. Lee, et al.; J. Biol. Chem. 279, 10564 (2004), Abstract; Full Text

Product Toolbox


Certificate of Analysis


By target:
By biological activity:
TLR Monoclonal antibody
TLR9 Monoclonal antibody
By catalog section:


Technical Service
Customer Service

Related Literature

Product Flyers
Antiviral Signaling
Antiviral Signaling
Download as PDF

Product Flyers
Key TLR Ligands and their Receptors
Key TLR Ligands and their Receptors
Download as PDF

All new literature pieces

Recommend this page

For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2019 Enzo Life Sciences, Inc.,