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Toll-like receptor 3 monoclonal antibody (40C1285.6) (FITC conjugate)

ALX-804-362F-C100 100 µg FITC 431.00 USD
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Product Specification

Alternative Name:TLR3, CD283
Immunogen:Synthetic peptide corresponding to aa 55-70 (VLNLTHNQLRRLPAAN) of human TLR3 (Toll-like receptor 3) cytoplasmic portion.
UniProt ID:O15455
Species reactivity:Human, Mouse
Applications:Flow Cytometry, IHC
Recommended Dilutions/Conditions:Flow Cytometry (cell surface, intracellular)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Protein G-affinity purified.
Formulation:Liquid. In 10mM TRIS containing 150mM sodium chloride, 0.05% BSA and 0.05% sodium azide.
Handling:Do not freeze. Protect from light.
Shipping:Shipped on Blue Ice
Short Term Storage:+4°C
Long Term Storage:+4°C
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Figure: Figure 2: Intracellular flow cytometric analysis of TLR3 on Ramos cells using using MAb to Toll-like Receptor 3 (40C1285) (FITC)  (Prod. No. ALX-804-362F) at 1µg/1x106 cells.
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Figure: Immunohistochemistry of human gut lumen (longitudinal section, transverse region) using MAb to Toll-like Receptor 3 (human) (40C1285.6) (Prod. No. ALX-804-362) at 10µg/ml. Data courtesy Dr. Elizabeth Furrie, University of Dundee.
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Product Literature References

Differential COX-2 induction by viral and bacterial PAMPs: Consequences for cytokine and interferon responses and implications for anti-viral COX-2 directed therapies: N.S. Kirkby, et al.; Biochem. Biophys. Res. Commun. 438, 249 (2013), Abstract; Full Text
Vitamin D3 down-regulates intracellular Toll-like receptor 9 expression and Toll-like receptor 9-induced IL-6 production in human monocytes: L.J. Dickie, et al.; Rheumatology (Oxford). 49, 1466 (2010), Application(s): Flow Cytometry, Abstract; Full Text
Biochemical and functional analyses of the human Toll-like receptor 3 ectodomain: C.T. Ranjith-Kumar, et al.; J. Biol. Chem. 282, 7668 (2007), Abstract; Full Text
Effects of single nucleotide polymorphisms on Toll-like receptor 3 activity and expression in cultured cells: C.T. Ranjith-Kumar, et al.; J. Biol. Chem. 282, 17696 (2007), Abstract; Full Text
Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils: C.K. Wong, et al.; Am. J. Respir. Cell Mol. Biol. 37, 85 (2007), Abstract;
Distinct poly(I-C) and virus-activated signaling pathways leading to interferon-beta production in hepatocytes: K. Li, et al.; J. Biol. Chem. 280, 16739 (2005), Abstract; Full Text
Regulation of the polymeric Ig receptor by signaling through TLRs 3 and 4: linking innate and adaptive immune responses: T.A. Schneeman, et al.; J. Immunol. 175, 376 (2005), Abstract;
Thyrocytes express a functional toll-like receptor 3: overexpression can be induced by viral infection and reversed by phenylmethimazole and is associated with Hashimoto’s autoimmune thyroiditis: N. Harii, et al.; Mol. Endocrinol. 19, 1231 (2005), Abstract;
Toll-like receptors-2, -3 and -4 expression patterns on human colon and their regulation by mucosal-associated bacteria: E. Furrie, et al.; Immunology 115, 565 (2005), Abstract;
The effect of innate immunity on autoimmune diabetes and the expression of Toll-like receptors on pancreatic islets: L. Wen, et al.; J. Immunol. 172, 3173 (2004), Abstract;
Subcellular localization of Toll-like receptor 3 in human dendritic cells: M. Matsumoto, et al.; J. Immunol. 171, 3154 (2003), Abstract;

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