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[pSer36]Shc monoclonal antibody (6E10)

 
ALX-804-358-C100 100 µg 552.00 USD
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Product Specification

Alternative Name:Shc transforming protein 1, SH2 domain protein C1, Shc1
 
Clone:6E10
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Synthetic peptide corresponding to aa 33-39 (E33LPpSPSA39) of shc phosphorylated at Ser36 conjugated to KLH.
 
UniProt ID:P29353
 
Species reactivity:Human, Mouse
Dog
 
Crossreactivity:Does not cross-react with non-phosphorylated shc.
 
Applications:WB
 
Recommended Dilutions/Conditions:Western Blot (1µg/ml for HRPO/ECL detection; recommended blocking buffer BPPT: 1% (w/v) BSA, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 2 x PBS). Immunoprecipitation of shc prior to detection with MAb to Shc (phosphorylated) (pSer36) (6E10) is recommended
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Positive Control:Included. (Prod. No. 840-033-1)
 
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
 
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
 
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
 
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 3 months.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 

Product Literature References

Amyloid-beta oligomerization is associated with the generation of a typical peptide fragment fingerprint: N. Rudinskiy, et al.; Alzheimers Dement. 12, 996 (2016), Application(s): SDS-PAGE and immunoblotting, Abstract;

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