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BimS/EL/L monoclonal antibody (14A8)

 
ALX-804-351-C100 100 µg 356.00 USD
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Product Specification

Alternative Name:BCL2L11
 
Clone:14A8
 
Host:Rat
 
Isotype:IgG2a
 
Immunogen:Recombinant mouse BimS.
 
UniProt ID:O54918
 
Species reactivity:Human, Mouse
Monkey
 
Specificity:Recognizes BimL, BimEL and BimS.
 
Applications:Flow Cytometry, ICC, IHC (PS), IP, WB
 
Recommended Dilutions/Conditions:Flow Cytometry (0.5-1µg/ml; overexpressing cell lines)
Immunocytochemistry (1-5µg/ml)
Immunohistochemistry (paraffin sections, 1-5µg/ml)
Immunoprecipitation (1-2µg/ml)
Western Blot (1-2µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein G-affinity purified.
 
Formulation:Liquid. In PBS containing 10mM sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Technical Info/Product Notes:As Bim expression is generally low in non-transfected cell lines, please take care when choosing positive controls. Examples of cell lines which express detectable levels of Bim include: B6.2.16.BW2 or KO52.DA20 (T cell lymphomas) or ABL5.8.1 (B cell lymphoma) or MCF-7s. It is generally necessary to use a high amount of lysate (e.g. 1 x 10^7 cells). When using tissue samples, it is generally necessary to use 1-5mg of total protein from an appropriate tissue source.
 
804-351(2)-n
Figures: IP/Western blot analysis of Bim expression in mouse (A) and human (C) cell lines. Bim proteins were immunoprecipitated from lysates of 107 cells with anti-BimS/EL/L MAb (clone 14A8) (Prod. No. ALX-804-351) and anti-BimL MAb (clone 5E5) (Prod. No. ALX-804-352). Western blot analysis was performed with clones 14A8 and 5E5 followed by the addition of biotinylated mouse anti-rat IgG2b antibody and HRP-conjugated mouse anti-rat IgG2a antibody as the respective secondary antibodies, and ECL detection. (B) depicts the ability of clone 14A8 to immunoprecipitate BimL and BimEL from lysates of spleen and thymus from normal mice, but not from Bim-deficient mice.
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Product Literature References

The role of BH3-only protein Bim extends beyond inhibiting Bcl-2–like prosurvival proteins.: D. Mérino, et al.; J. Cell. Biol. 186, 355 (2009), Abstract;
Constitutive association of the proapoptotic protein Bim with Bcl-2-related proteins on mitochondria in T cells: Y. Zhu, et al.; PNAS 101, 7681 (2004), Abstract; Full Text
BH3-only Bcl-2 family member Bim is required for apoptosis of autoreactive thymocytes: P. Bouillet, et al.; Nature 415, 922 (2002), Abstract;
Induction of BIM, a proapoptotic BH3-only BCL-2 family member, is critical for neuronal apoptosis: G.V. Putcha, et al.; Neuron 29, 615 (2001), Abstract;
The proapoptotic BH3-only protein bim is expressed in hematopoietic, epithelial, neuronal, and germ cells: L.A. O'Reilly, et al.; Am. J. Pathol. 157, 449 (2000), Abstract; Full Text
Proapoptotic Bcl-2 relative Bim required for certain apoptotic responses, leukocyte homeostasis, and to preclude autoimmunity: P. Bouillet, et al.; Science 286, 1735 (1999), Abstract;
The proapoptotic activity of the Bcl-2 family member Bim is regulated by interaction with the dynein motor complex: H. Puthalakath, et al.; Mol. Cell 3, 287 (1999), Abstract;
Rapid hybridoma screening method for the identification of monoclonal antibodies to low-abundance cytoplasmic proteins: L.A. O'Reilly, et al.; Biotechniques 25, 824 (1998), Abstract;

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